摘   要

厌氧氨氧化工艺是以厌氧氨氧化菌群为主而进行厌氧氨氧化脱氮反应，该工艺具有高效低耗优势，近年成为废水生物处理领域的热点。但该工艺也易受外部如抗生素等各类环境因子干扰，本文研究了抗生素土霉素对厌氧氨氧化工艺的影响。实验结果表明：经过两个土霉素添加阶段（1和2 mg L-1），实验组R1反应器的脱氮效率由94。3%降至50。3%，而对照组R0脱氮效率稳定在85%以上；土霉素添加实验组的厌氧氨氧化菌群活性由347。7±5。3 mg N g-1VSS d-1降为65。1±2。1 mg N g-1VSS d-1，血红素c含量由3。86±0。03 μmol g-1VSS降为1。94±0。04 μmol g-1VSS，而对照组的活性和血红素c含量均增加。根据qPCR结果分析，土霉素添加实验组中厌氧氨氧化菌数量呈下降趋势，其相对丰度由43。1%降至32。9%，而对照组数量及相对丰度均增加。无论从生理生化特性还是从厌氧氨氧化菌数量及相对丰度分析，土霉素的添加都对厌氧氨氧化工艺表现出抑制作用。90467

The  anaerobic  ammonium  oxidation  (anammox)  process  refers  to the  of ammonium to nitrogen gas using nitrite as the electron acceptor in the absence of oxygen。 The anammox process has advantages, such as high efficiency  and low  energy  consumption,  which  becomes hot spot of wastewater biological treatment。 However, anammox process is sensitive to environmental conditions like antibiotics。 The paper studied the effect of oxytetracycline on anammox process。 After two stages of oxytetracycline adding (1, 2 mg L-1), Anammox nitrogen removal efficiency was decreased from 94。3% to 50。3%, while it kept stable at above 85% in the control reactor。 In the experimental reactor, the specific Anammox activity was reduced from 347。7±5。3 to 65。1±2。1 mg N g-1VSS d-1, and heme c content was decreased from 3。86±0。03 to 1。94±0。04 μmol g-1VSS, while both of specific Anammox activity and heme c content increased in the control reactor。 Based on qPCR results, 

the number of anaerobic ammonia oxidizing bacteria (AnAOB) declined gradually and the relative abundance ranged from 43。1% to 32。9% with the addition of oxytetracycline, which were just the opposite for the control reactor。 The analysis of physiological and biochemical indexes and AnAOB quantity 

suggested an inhibitory toxicity of oxytetracycline to anammox process。 

毕业论文关键词：厌氧氨氧化菌； 土霉素； 脱氮效率； 抑制；

Keywords: anaerobic ammonium oxidation microbial community; oxytetracycline; tAnammox nitrogen removal efficiency; inhibitory

目  录

摘   要 2

实验仪器源Q于W优H尔J论K文M网WwW.youeRw.com 原文+QQ75201.,8766 和材料 4

1。1实验仪器 4

1。2菌种 4

1。3模拟废水 4

方法 4

2。1反应器运行 4

2。2指标测定 5

2。3比厌氧氨氧化活性（VSS）分析 5

2。4 样品采集 5

2。5 DNA提取 5

2。6 PCR扩增 5

2。7 目的条带回收 6

2。8 目的片段连接 6

2。9 连接产物的热击转化 6

2。10 蓝白斑筛选及质粒回收