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摘要以壳聚糖（CTS）为原料，2,3-环氧丙基三甲基氯化铵(GTMAC)为改性剂做季铵化改性来制备取代度较高的2-羟丙基三甲基氯化铵壳聚糖(HTCC) 。以聚醚砜超滤膜(PES)为支撑层，环氧氯丙烷(ECH)为交联剂,制备出荷正电的HTCC/PES复合纳滤膜。通过红外光谱(FTIR)、X-射线衍射(XRD)、扫描电镜(SEM)等方法对壳聚糖（CTS）以及壳聚糖季铵盐（HTCC）的形态结构进行表征和分析，采用化学还原法来制备HTCC-Ag 铸膜液，采用涂敷交联反应法制备HTCC-Ag复合纳滤膜。通过扫描电镜法对PES膜、HTCC/PES膜HTCC-Ag/PES膜的表面结构进行了观察，并着重研究膜的抗菌性能。18918
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结果显示，CTS分子链中氨基(-NH2)上的氢原子已被季铵盐基团部分取代，通过扫描电镜可看出纳米银颗粒成功地负载到膜表面上。在还原剂硼氢化钠和柠檬酸三钠的作用下，银离子被还原成纳米银粒子核。且从扫描电镜图可看出，纳米银颗粒之间没有明显的团聚现象，粒子的聚集只发生在局部相区内。抑菌圈实验表明三种膜对E.coli Rosetta和E.coli DH5a以及Bacillus subtilis的抗菌能力大小依次为: HTCC-Ag/PES膜>HTCC/PES膜>PES膜,并且HTCC-Ag/PES膜对革兰氏阴性菌E.coli的抗菌能力优于革兰氏阳性菌 Bacillus subtilis。
关键词： 壳聚糖，2-羟丙基三甲基氯化铵壳聚糖，环氧氯丙烷，荷正电复合纳滤膜，抗菌性
毕业设计说明书（毕业论文）外文摘要
Title Chitosan quaternary ammonium salt preparation and antibacterial
properties of nano silver/PES antimicrobial film
Abstract
With a molar feed ratio of 1:4 of chitosan (CTS) and (GTMAC) preparation of 2 - hydroxypropyl trimethyl chitosan chloride (HTCC) film.positively charged composite nanofiltration membrane was prepared by using HTCC as the selective layer, PES UF membrane as support layer and epichlorohydrin (ECH) as cross-linking reagent. The prepared HTCC was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), on membrane performance were studied and the membrane was characterized by SEM .
The results show that the hydrogen atoms in the molecular chain CTS amino (-NH2) substituents on the quaternary ammonium salt group has been part of the quaternary ammonium salt of chitosan and the higher the degree of substitution corresponding to the corresponding antibacterial performance gradually. The optimum preparation conditions of HTCC-Ag/PES hybrid membrane were as follows:1.0wt% HTCC casting solution, mole ratio of material nCTS: nGTMAC=1:4,cross-linked with 1.9wt% ECH and 0.3g KOH in 50mL ethanol for 21-22h and drying temperature 50 ℃ was found to have optimum performance.Additionally, the antibacterial assays indicated that the antibacterial ability of tested membranes against E.coli Rosetta, E.coli DH5a and Bacillus subtilis was HTCC-Ag/PES hybrid membrane > HTCC membrane > CTS membrane, and these membranes showed the greater antibacterial activity against Gram-negative bacteria E.coli than Gram-positive bacteria Bacillus subtilis.
Key words: Chitosan, HTCC-Ag/PES, ECH, positively charged composite NF membrane, membrane antibacterial
目 录 I
1 引言 1
1.1 壳聚糖的改性 1
1.1.1 季铵化壳聚糖 1
1.1.2 烷基化壳聚糖 2
1.1.3 酰基化壳聚糖 2
1.2 HTCC的应用 3
1.2.1 抗菌性 3
1.2.2 生物相容性 3
1.2.3 成膜性 3
1.3 研究内容及意义 3
1.3.1 研究内容 3
1.3.2 研究意义 4
2 壳聚糖季铵盐HTCC的制备和表征 5
2.1 实验部分 5