摘要利用电化学沉积的方法将染料儿茶酚紫 (pyrocatechol violet, PCV) 沉积在单壁碳纳米管 (single wall carbon nanotubes, SWCNTs) 修饰的玻碳电极 (glassy carbon electrode, GCE)表面，构筑了一种新型的无酶电化学传感器（PCV/SWCNTs/GCE）。电化学沉积儿茶酚紫的最佳条件：电位控制在0  0.9 V vs. SCE之间，扫描速率为20 mV·s-1，扫描圈数为20，电解液为pH = 3且含有1 mmol·dm-3儿茶酚紫的0.1 mol·dm-3磷酸缓冲溶液。以β-烟酰胺腺嘌呤二核苷酸(β-Nicotinamide adenine dinucleotide, NADH) 来考察PCV/SWCNTs/GCE的电化学催化能力。通过循环伏安法、安培响应法等电化学方法，推断PCV/SWCNTs/GCE电化学催化NADH的机理及控制过程。PCV/SWCNTs/GCE具有快速的电子传递能力，对NADH表现出优异的电化学催化性能。在0.17 V vs.SCE时，可以实现对NADH的快速、灵敏的检测。 检测线性范围为5.26×10-7 ～ 2.04×10-5 mol·dm-3，最低检测限为5.2×10-7 mol·dm-3 (信噪比为3)，响应时间 < 4 s，检测灵敏度为317.7 mA·M-1·cm-2。64754

毕业论文关键词  碳纳米管  儿茶酚紫  电化学传感器  β-烟酰胺腺嘌呤二核苷酸

毕业设计说明书（论文）外文摘要

Title Fabrication of electrochemical sensor based on pyrocatechol violet-single wall carbon nanotubes and its applications.

Abstract

A stable electroactive film of pyrocatechol violet (PCV) was electrodeposited on the surface of glassy carbon electrode modified with single wall carbon nanotubes (SWCNTs) to construct a novel electrochemical sensor (PCV/SWCNTs/GCE). The optimal conditions of PCV electrodeposition were that the scan potential range was controlled between 0 and 0.9 V vs. SCE for 20 cycles with the scan rate of 20 mV·s-1, and the electrolyte was 0.1 mol·dm-3 phosphate buffer (pH = 3) containing 1.0 mmol·dm-3 PCV. β-Nicotinamide adenine dinucleotide (NADH) was used to evaluate the electrocatalytic activity of PCV/SWCNTs/GCE. The reaction mechanism and control process were investigated by cyclic voltammograms and amperometry. PCV/SWCNTs/GCE showed an excellent electrocatalytic activity for NADH at 0.17 V vs.SCE, and the fast response was proportional to NADH concentration in the range of 5.26×10-7 ～ 2.04×10-5 mol·dm-3 with a detection limit and sensitivity of 5.2×10-7 mol·dm-3 (S/N = 3) and 317.7 mA·M-1·cm-2, respectively.

Keywords  carbon nanotubes; pyrocatechol violet; electrochemical sensor; β-Nicotinamide adenine dinucleotide

目   次

1  绪论 1

1.1  引言 1

1.2  电化学传感器简介 1

1.3  生物传感器简介 1

1.4  新功能纳米材料 2

1.5  基于碳纳米管复合材料的制备 3

1.6  基于碳纳米管复合材料的形貌表征和电化学测试 5

1.7  基于碳纳米管复合材料生物电极的构筑 6

2.1  引言 8

2.2  实验部分 9

2.3  结果与讨论 10

2.4  本实验的创新性与优缺点 28

2.5  小结 28

结  论 30

致  谢 31

参考文献