摘要: 以溶液还原法对聚乙烯亚胺(PEI) 和Cu2+ 形成的配合物进行还原,制备出联结有聚乙烯亚胺的出铜纳米颗粒，基于透明质酸受体在正常细胞和肿瘤细胞表面表达出来的差异性，然后用透明质酸（HA）修饰,得到透明质酸修饰的接枝了聚乙烯亚胺的铜纳米颗粒（即Cu-PEI-HA纳米颗粒）。使用扫描电子显微镜，动态光散射，红外光谱等分析仪器得到Cu纳米颗粒的结构、组成和形貌，制备出粒径在100nm左右的纳米铜颗粒。透明质酸修饰的Cu-PEI-HA颗粒作为罗丹明B的药物运输载体，室温载药12h，平衡吸附量稳定在0.23mg/mg左右。Cu纳米颗粒具有一定的载药能力。70968

毕业论文关键词:Cu纳米颗粒；透明质酸；聚乙烯亚胺；复合物

Development and Performance Test of Functionalized Inorganic Nanoparticles of Hyaluronic Acid

Abstract: The complexes of polyethylene mine (PEI) and Cu2 + were reduced by solution reduction method, and the metal Nano-copper particles with polyethylene mine were prepared. The hyaluronic acid receptor was expressed on the surface of normal cells and tumor cells and then modified with hyaluronic acid (HA) to obtain hyaluronic acid-modified Nano-copper particles grafted with polyethylene mine (i.e., Cu-PEI-HA nanoparticles). The structure, composition and morphology of Cu nanoparticles were obtained by scanning electron microscopy, dynamic light scattering and infrared spectroscopy. The nanometer copper particles with particle size of about 100 nm were prepared. The hyaluronic acid-modified Cu-PEI-HA particles were used as drug transport carriers for Rhoda mine B, and the equilibrium adsorption capacity was stable at about 0.23 at room temperature for 12 h. Cu nanoparticles have a certain drug loading capacity.

Key words: Cu particles; hyaluronic acid; polyethyleneimine; complexes

目录

1. 绪论 1

1.1铜纳米颗粒 1

1.1.1 铜纳米颗粒的制备方法 1

1.2 聚乙烯亚胺 4

1.2.1 聚乙烯亚胺的性质 4

1.2.2 聚乙烯亚胺的研究现状 5

1.3 透明质酸 6

1.3.1 透明质酸的发现 6

1.3.2 透明质酸在医学领域的应用 7

1.4本课题研究及意义 9

2.实验部分 10

2.1 实验试剂和仪器 10

2.1.1 实验试剂 10

2.1.2 实验仪器 10

2.2 实验方法 10

2.2.1 Cu颗粒的制备 10

2.2.2 罗丹明B的负载 11

2.2.3 SEM测定 11

2.2.4 纳米粒子的红外光谱检测 11

2.2.5 紫外光谱检测 12

3. 结果与讨论 12

3.1 纳米铜微粒的表征 12

3.1.1 粒径分析 12

3.1.2形貌分析 13

3.1.3 红外光谱分析 14

3.2 罗丹明B标准曲线 15

3.2.1 罗丹明B的负载数据 16

4. 小结 18

致谢