响应面法优化富硒平菇发酵工艺条件
关键词：富硒平菇；单因素试验；响应面法；发酵；优化
The Response Surface Method To Optimize The Rich Selenium Mushroom Fermentation Conditionsditions
Abstract: Mushroom spawn provided by Zhoukou Academy were tested for its resistance to selenium range, single factor experiment and response surface methodology Box-behnken Design (BBD) optimization test. 3,3 '- diaminobenzidine hydrochloride method was applied to determine the selenium content of mushroom under the conditions of different components and proportion. The results of single factor experiment shows as follows: the optimum value of appropriate selenium content for mushroom mycelium growth is 250 mg / kg training materials, the best value of wheat bran content is 240 g / kg cottonseed hulls, the optimum value of sucrose content is 24 g / kg cottonseed hulls. The optimum Se mushroom growth formula is determined by response surface experiment as follows: wheat bran 163.60g/kg cottonseed hulls, sucrose 15.08 g / kg cottonseed hulls, selenium 398.83 mg / kg training materials. Under optimized conditions, the selenium content 2.84543 mg / kg agreed closely with the predicted value 2.81547mg/kg. It indicates predictive model is applicable and reliable, so it can be applied to optimize the fermentation conditions of Se mushroom.This research for the rich selenium mushroom provides theory basis for the scale of production, has a certain reference value.
Key Words: The rich selenium mushroom; Single Factor Experiment; The response surface method; Cultivation Conditions; Optimization
目 录
摘 要. 1
Abstract 1
引言 2
1.材料与方法 2
1.1试验材料 2
1.2试验方法 3
2.结果与分析 5
2.1硒标准曲线的绘制 5
2.2单因素试验的结果及分析 6
2.3响应面法试验的结果及分析 8
2.4验证性试验的结果分析 11
3.结论与讨论 12
参考文献 12
致谢 14
响应面法优化富硒平菇发酵工艺条件 引言
硒是人体必需的微量元素，与人类和动物的健康密切相关，已证明在某些地区流行的克山病、大骨关节病，牲畜所患的白肌病与缺硒有关，通过补硒可使疾病得到控制。研究发现低硒与心血管病、肝病、癌症的发病有关[1-4]。人体内的硒大多从平时的食物中获得，但食物中硒的含量通常比较低，很难满足人体的需要。而无机硒的毒性通常又比较高，所以能将无机硒转化为有机硒的方法得到了国内外学者的关注[5-8]。平菇甘、温，具有追风散寒、舒筋活络的功效，可用于治疗腰腿疼痛、手足麻木、筋络不通等病症。平菇含丰富的营养物质，蛋白质含量高，氨基酸种类齐全，矿物质丰富。硒蛋氨酸（Se-Mef）库是动物体内最重要的硒代谢库之一，而平菇中氨基酸种类齐全，所以富硒平菇的研究有很重要的意义[9]。在国外已有很多国家研究富硒平菇并且实现了商业化生产。但是，国内有关富硒平菇发酵生产的报道还不多，对最适宜的培养条件研究的还不是很清楚，实现规模生产还不太现实，因此研究富硒平菇显得尤为必要。本研究选用农科院提供的平菇菌种，对其富硒能力进行研究，优化富硒平菇的发酵工艺条件，为进一步的研究提供参考。
下一篇：黑豆豆芽异黄酮微波提取工艺研究