番茄晚疫病抗性基因的电子定位和结构分析
关键词：番茄；晚疫病；氨基酸序列；生物信息学
Electronic positioning and structural analysis of tomato
late blight resistance genes
Abstract: The protein sequences of late blight resistance genes of tomato were received from the NCBI database, using bioinformatics methods to analysis the Phylogenetic tree, genome positioning and the structure of the genes, analysis and predict the physicochemical property, amino acid composition, secondary structure and tertiary structure et al. Phylogenetic tree indicated that these proteins can be pided into 7 subtribes, and they had high conservatism with each other. Genome positioning showed that 81 late blight resistance genes distributed on the different sections of 12 chromosomes of tomato, and the number of genes on each chromosome was different. The study also found that the number of amino acids, the pI, grand average of hydropathicity et al were different. The predictive results of secondary structure found that the main compositions of 81 amino acid sequences were α-helix and random coil, and the tertiary structure of all 81 amino acid sequences was similar. These results will provide very important theory on the resistance study of tomato late blight.
目 录
摘 要 1
引 言 2
1.材料和方法 3
1.1数据库的检索 3
1.2序列分析 3
1.3番茄晚疫病抗性蛋白结构分析 3
1.3.1番茄晚疫病抗性蛋白一级结构分析 3
1.3.2番茄晚疫病抗性蛋白二级结构分析 3
1.3.3番茄晚疫病抗性蛋白三级结构分析 3
2.结果与分析 3
2.1番茄晚疫病抗性基因家族成员的挖掘 3
2.2番茄晚疫病抗性蛋白的系统发生树分析 3
2.3番茄晚疫病抗性基因的基因组定位 6
2.4氨基酸组成以及理化性质分析 6
2.5蛋白质二、三级结构预测分析
2.5.1蛋白质二级结构预测分析
2.5.2蛋白质三级结构预测分析
2.6番茄晚疫病抗性基因的定位与基本结构分析
3.结论与讨论
参考文献
致 谢
番茄晚疫病抗性基因的电子定位和结构分析引言
番茄（Lycopersicon esculentum Mill）是被子植物门、双子叶植物纲、茄目、茄科、茄属物种。番茄起源于南美的秘鲁、厄瓜多尔、玻利文亚，在南美西部安第斯山脉的狭长地带均有番茄野生种存在。根据Jenkins(1948)考证，栽培番茄最初起源于秘鲁的矮克度(Ecuador)，其祖先是樱桃番茄(L．esculentum var Cerasiforme)。栽培番茄在16世纪末或17世纪初的明万历年间传入我国。最早记载是在佩文斋的《广群芳谱》(1708年)上，使用“番柿”这一名称，主要用于观赏，据园艺学家吴耕民教授考证，我国番茄栽培始于本世纪初，尚不足百年。现在已经被作为食用蔬果被全球性广泛种植。
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