摘要拟南芥DWF5基因被报道编码了植物激素油菜素内脂BRs的合成酶，其基因功能缺失会导致BRs合成受阻出现缺乏症，但其对植物生长发育的作用和机制尚缺乏深入研究。本研究以拟南芥T-DNA插入失活突变体dwf5为对象，以野生型植株为对照，考察了AtDWF5基因功能缺失对拟南芥生长发育的影响。研究结果表明DWF5基因突变导致幼苗生长受阻，根长缩短，鲜重减轻；DWF5基因突变导致成苗生长受阻，植株矮小，分蘖数增加、长角果长度缩短，其种子重量较轻。期望通过研究明确AtDWF5基因对拟南芥生长发育的影响，并为进一步揭示其调控机制提供新的数据和参考。88140

Abstract Arabidopsis DWF5 gene were reported to code synthetase of plant hormones BRs, the loss of which will lead to deficiency in BRs synthesis。 However, its effect and mechanism in regulating plant growth still remains unclear。 In this study, Arabidopsis T–DNA inserted mutant of dwf5 were analyzed, with the wild type plants as controls, to investigate the impact on plant growth of this AtDWF5 gene。 Our data showed that mutation of DWF5 gene could lead to shortened roots and retarded growth of seedlings。 Our data also showed that mutation of DWF5 gene could lead to smaller and lower plants, increased tillers, shortened siliques and retarded growth。 We expectated to make clear the influence of AtDWF5 gene on the growth and development of Arabidopsis, and provides new data for further study on its regulatory mechanism。

毕业论文关键词：拟南芥；DWF5；生长发育；野生型；突变体

Keyword: Arabidopsis thaliana; DWF5; growth and development; wild type; mutant

目    录From~优E尔L论E文W网wWw.YoUeRw.com 加QQ7520.18766

目录 3

引言 4

1 材料与方法 5

  1。1 材料 5

  1。2 主要试剂和仪器 5

  1。3 拟南芥种植 5

    1。3。1 种子表面消毒和破除休眠 6

    1。3。2 幼苗的萌发和转移 6

    1。3。3 拟南芥的培养 6

  1。4 纯合突变体中DWF5基因表达检测 7

    1。4。1 植物RNA的提取 7

    1。4。2 逆转录获取cDNA 7

    1。4。3 RT-PCR检测基因表达量 7

  1。5 拟南芥幼苗表型观测 8

    1。5。1 根长统计 8

    1。5。2 植株鲜重统计 8

  1。6 拟南芥成苗表型观测 8

    1。6。1 植株鲜重统计 8

    1。6。2 分蘖数统计 8

    1。6。3 株高统计 8

    1。6。4 长角果长度统计 8

    1。6。5 种子重量及颜色统计 9

2 实验结果 9

  2。1纯合dwf5突变体基因表达检测 9

  2。2 DWF5基因突变导致幼苗生长受阻 10