富氢水对玉米缺铁适应性的影响及其机理研究
毕业论文关键词：玉米；缺铁；富氢水；光合机构；
Effects of hydrogen enriched water on iron deficiency adaptability of maize and its mechanism Student majoring in biotechnology
Abstract：In our study, we used maize varieties ND108 as materials, that were grown in Hoagland's solution with monofactor controlled solutions. We study the function of HRW under the iron deficiency by measuring plant growth, chlorophyll content, photosynthetic gas exchange parameters, chlorophyll fluorescence parameters , Fe content and active Fe content. The results showed that maize seedling grew weaker and chlorophyll content reduced significantly. We find that 50% concentration of HRW had a significant concentration-dependent effect on biomass and chlorophyll content under the iron deficiency. The biomass and pigment content of 50% HRW treatment increased, and the content of active iron and total iron increased, and the Fv / Fm and PI value, the activity of PSII reaction center was restored and the electron transport rate was increased. At the same time, the photosynthetic gas exchange parameters were improved by HRW treatment, that is, the stability of photosynthetic apparatus of maize seedlings was enhanced under iron deficiency stress.
Key words: Maize; Iron Deficiency; Hydrogen-rich water; Photosynthetic apparatus
目录
摘要 1
关键词： 1
1 材料与方法 2
1.1富氢水的制备 3
1.2 材料培养及富氢水处理 3
1.3 MDA和H2O2的测定 3
1.4 SOD，CAT，APX，GR，POD抗氧化酶活性的测定 3
1.5叶片光合气体交换参数的测定 4
1.6 叶绿素荧光参数的测定与分析 4
1.7 数据处理及统计方法 4
2 结果与分析 5
2.1 HRW对缺铁胁迫下玉米幼苗生长和叶绿素含量的影响 5
2.2 HRW对缺铁胁迫下玉米幼苗铁含量的影响 6
2.3 HRW对缺铁胁迫下玉米幼苗叶绿素荧光参数的影响 6
2.4 HRW对缺铁胁迫下玉米幼苗光合机构稳定性的影响 9
2.5 HRW对缺铁胁迫下玉米幼苗抗氧化酶活性的影响 11
2.6 HRW对缺铁胁迫下玉米幼苗MDA和H2O2含量的影响 12
3 讨论 13
致谢 15
参考文献 15
富氢水对玉米缺铁适应性的影响及其机理研究
铁是植物生长发育所必须的一种微量元素。由于铁的溶解度极低，使得在土壤中铁的有效含量也低，特别是在碱性土壤中，从而导致植物对铁的吸收利用受到限制（Wang et al. 2015）。土壤中铁的有效含量低，使得植物在进化中需不断提高对铁的吸收机制，这样才能保证植物的正常生长及发育。同时，植物在长期的进化过程中，已逐渐形成了一些适应环境铁缺乏的机制。主要包括适应性机理和非适应性机理两种。适应性机理明显受体内铁营养状况所调节 ,适应性机理又划分为机理I(strategy I)和机理II ( strategy II)植物，前者包含双子叶和非禾本科单子叶植物，后者为禾本科单子叶植物。植物在遭受缺铁胁迫时，其表观主要表现为叶片发黄，植株矮小，同时叶绿素含量降低，光合速率下降，最终导致农作物的产量下降。因此，研究如何保护植物来抵抗缺铁胁迫对以后的农业发展具有重大意义。