怀牛膝皂苷提取工艺及抗氧化活性研究
毕业论文关键词：怀牛膝；皂苷；微波提取；抗氧化活性
Study on the Extraction Technology of Saponins from Achyranthes Bidentata Bl. and its Antioxidant Activities
Abstract: To explore the influence factors impacting extraction rate of saponins from Achyranthes bidentata Bl, the optimal extraction conditions using microwave- assisted extraction were optimized by single factor and L9(34) orthogonal experiment, which included the solid-liquid ratio, ethanol concentration, microwave-heating time and microwave power from the point of extraction rate of saponins. At the same time, the saponins content and antioxidant activities of wild root, flowers and stems-leaves was detected. The results showed that the optimal conditions were as follows: the 1:30 ratio of solid-liquid, ethanol concentration was 70%, extracting for 4 min and microwave power was high fire. The saponins extraction rates of different parts of Achyranthes bidentata Bl. was obtained under these conditions as follows: 0.523% of root, 0.420% of wild root, 0.082% of flowers, 0.235% of stem-leaf. It was also found that the extracts of Achyranthes bidentata Bl. had different antioxidant activities. The clearance rates of superoxide anion radical in different parts of Achyranthes bidentata Bl. were as follows: 81.32% of root, 67.36% of wild root, 11.06% of flowers and 28.81% of stem-leaf. The clearance rates of hydroxyl radical in different parts of Achyranthes bidentata Bl. were as follows: 60.23% of root, 45.46% of wild root, 9.48% of flowers, 32.23% of stem-leaf.
Key words: Achyranthes bidentata Bl; Saponins; Microwave extraction; Antioxidant
目 录
摘 要 1
引言 2
1实验部分 2
1.1实验所用材料、试剂及仪器 2
1.2实验方法 3
1.2.1皂苷测定 3
1.2.1.1标准液的配制 3
1.2.1.2皂苷标准曲线的绘制 3
1.2.1.3皂苷提取工艺流程 3
1.2.1.4皂苷提取率的测定 3
1.2.2单因素实验 4
1.2.3正交实验 4
1.2.4抗氧化活性 4
1.2.4.1怀牛膝提取液对超氧阴离子自由基清除活性 4
1.2.4.2怀牛膝提取液对羟自由基清除活性 4
2结果与讨论 4
2.1单因素实验 4
2.1.1料液比的选择 4
2.1.2乙醇浓度的选择 5
2.1.3微波时间的选择 5
2.1.4微波功率的选择 6
2.2正交实验结果 7
2.3抗氧化实验结果 8
2.3.1怀牛膝提取液对超氧阴离子自由基清除活性 8
2.3.2怀牛膝提取液对羟自由基清除活性 8
3结论 9
参考文献 9
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