摘要：植物通过分泌根系分泌物调节土壤微生物群落多样性，改善土壤的生态环境，而根际微生物的活动也会改变植物根系分泌物。本研究通过气相色谱质谱联用技术（GC-MS）和实时荧光定量PCR（qPCR）技术，通过分根实验收集了经过导病土壤微生物、抑病土壤微生物处理和霍格兰营养液（对照）处理的香蕉根系分泌物，比较了不同处理下香蕉根系分泌物组分差异；通过向导病和抑病土壤中加入不同处理的香蕉根系分泌物进行室内培养，再接种等浓度病原菌孢子悬液后比较不同处理病原菌的数量。研究结果表明，不同处理的香蕉根系分泌物组分有很大差异，不同处理下土壤微生物对抵御外源病原菌入侵的反馈不同。研究初步探索了在香蕉根系分泌物介导下的土壤与微生物的相互作用，对研究土壤微生态系统功能、调控植物根际的生态过程以及对促进农业可持续发展等具有重要的指导意义。43578

毕业论文关键词：抑病型土壤；香蕉枯萎病；根系分泌物；根际微生物生态

Study on banana root exudate and Rhizosphere microbes induced by disease-suppressive soil

Abstract: Plant regulates soil microbial community persity by secreting root exudates to improve soil ecological environment. The change of rhizosphere microbial ecological environment depends on the type and quantity of root exudates. In this study, firstly, split-root container was used to collect banana root exudates treated by conducive soil slurry suppressive soil slurry and ck respectively. GC-MS were used to identified the difference of root exudates caused by different treatments. Secondly, conducive soil and suppressive soil were treated by the different root exudates for half a month, and then we investigate the total copies of soil bacteria, fungi and fusarium with qPCR techniques after 105 (dry soil) pathogenic spore added to the soil half a month later. Principal components analysis showed that the treatments were well separated with significant difference between each other. Soil microbes have different feedback against external pathogen invasion. This study showed the interaction of plant-soil-microbes under root-mediated secretion, which is of great significance to reveal the function of soil micro-ecosystem and regulate the ecological process of plant rhizosphere and promote the sustainable development of agriculture.

Key words: Suppressive soil; Fusarium wilt of banana; Root exudates; Rhizosphere microbial community

目  录

摘要1

关键词1

Abstract1

Key words1

引言（或绪论）1

1材料与方法2

1．1  实验材料 2

1.1.1 供试土壤2

1.1.2 供试作物2

1.1.3 供试菌种2

1.1. 4  供试培养基试剂2

1.1.5仪器2

1.2  实验方法3

1.2.1霍格兰营养液制备3

1.2.2土壤悬液制备3

1.2.3孢子悬液制备3

1.2.4根系分泌物实验3

1.2.5植株培养3

1.2.6根系分泌物的收集分析3

1.2.6.1代谢物萃取3

1.2.6.2代谢物衍化4

1.2.6.3上机检测4

1.2.6.4数据处理4

1.3不同土壤微生物根系分泌物实验4

1.3.1实验设计4

1.3.2提取土壤DNA4

1.3.3实时定量PCR5

1.4数据统计与分析5

2结果与分析 5

2.1 气相色谱-质谱分析6

2.1.1样本信息6

2.1.2组分热图分析7

2.2病原菌入侵分析8

2.2.1不同处理下细菌丰度分析8

2.2.2不同处理下真菌丰度分析9

2.2.3不同处理下尖孢镰刀属丰度分析9

2.2.4不同处理下尖孢镰刀属丰度占总真菌丰度分析10