摘要实验研究干旱胁迫下菊科种子萌发的状况,采用莴苣和野菊花这两种菊科的种子作为研究材料,利用浓度不同的聚乙二醇溶液(PEG溶液)模拟不同的干旱情况,设置PEG溶液的浓度梯度如下:0g/L、25g/L、50g/L、75g/L、100g/L、150g/L和200g/L,结果表明,当PEG浓度从0g/L增加到75g/L时,这两种种子的萌发率都呈现明显的上升趋势;而PEG浓度从75g/L增加到200g/L时,这两种种子的萌发率不断降低。其中,当PEG浓度达到75g/L时,莴苣种子和野菊花种子的发芽率达最大,分别为90.76%和84.50%;而PEG浓度达到200g/L时,莴苣种子和野菊花种子发芽率最低,分别为32.87%和3.50%。接着,对这两种种子萌发过程的形态进行观察,发现在去离子水(即PEG浓度为0g/L)和高浓度PEG溶液中,野菊花种子萌发时先进行发育的都是子叶,而莴苣种子在去离子水中先进行发育的是胚根,在高浓度PEG溶液中先进行发育的是子叶。初步表明莴苣种子在胁迫条件下,可进行一种适应性成长。69932
毕业论文关键词: 莴苣 野菊花 种子萌发 聚乙二醇 干旱胁迫
Seed germination mechanism research
Abstract:
In this experiment, two types’ seeds of Asteraceae (Dendranthema indicum and Lactuca sativa) were used as the material to operate on the seed germination under the simulated drought stress of different concentrations of PEG. , The result displayed that with the concentration of PEG increasing , the rate of emergence of the D. indicum and L. sativa was increased,while it was decreased when the concentration was set over 75 g/L .Moreover, the germination process of D. indicum and L. sativa seeds were quite different in deionized water: seeds of L. sativa performed radicle growth first but seeds of D. indicum performed cotyledon development first among their germination. Furthermore, the germination process of L. sativa seeds in PEG of high concentration were differ from that in deionized water: seeds of L. sativa performed radicle growth first in deionized water but performed cotyledon development first in PEG of high concentration among their germination.
Key words: Lactuca sativa Dendranthema indicum seed germination PEG droughty stress
目录
摘要 Ⅰ
Abstract: Ⅱ
目录 Ⅲ
引言 4
1 材料与方法 5
1.1供试材料及处理 5
1.2 实验方法 5
1.3 测定内容和方法 5
2 结果与分析 6
2.1 水势对两种菊科植物种子萌发率的影响 6
2.2 不同PEG处理液对菊科种子萌发进程的影响 7
2.3去离子水中莴苣与野菊花种子萌发比较 9
3.讨论 10
3.1水势与种子萌发 10
3.2莴苣种子萌发过程与水势的关系 10
3.3种子大小与发芽过程的关系 11
参考文献 12
致谢 13
引言
菊科是双子叶植物的一个大类,是世界种子中植物进化程度最大的科之一,菊科植物突出表现在形态结构方面先进且对环境有较强的适应能力,无论是在种类的数量上还是分布地区上均位居世界种子植物之冠[1]。在双子叶植物当中菊科当之无愧的是最多的一个科了,总共有13族,约1300属,25000余种,热带地区较少,分布于全世界,是很重要的药效植物,此次将野菊花和莴苣作为实验研究的对象。