摘要：本文以经干燥灭菌的银杏叶为材料，选用紫外分光光度法测定银杏叶中黄酮含量，用大孔吸附树脂分离纯化提取液。比对料液比、提取溶剂、浸提时长、浸提温度的单因素实验研究对银杏黄酮提取效率的影响，比较AB-8，D101两种大孔树脂对银杏黄酮的吸附效果，得出实验提取银杏黄酮的最佳工艺和最佳纯化树脂。以D101型大孔树脂对通过最佳工艺提取的银杏黄酮进行分离纯化得到精制品。实验得到提取的最佳工艺为：以50%乙醇为提取溶剂，在料液比1:30的情况下80摄氏度提取5小时。用D101型大孔树脂纯化后真空浓缩再冷冻干燥得率为3%。76463

毕业论文关键词：银杏叶、黄酮、最佳工艺

Study on optimization of extraction technology of flavonoids from Ginkgo biloba leaves

Abstract: In this paper, the dry sterilization of ginkgo biloba for the material, using ultraviolet spectrophotometric method determination of flavonoid in the ginkgo biloba, with macroporous adsorption resin purification extract。 Investigation is expected to 

liquid ratio, extraction solvent, extraction time, extraction temperature of single factor experiment and research on the effects of ginkgo flavonoids extraction efficiency,leaching extraction time, extraction temperature single factor experiment research on Ginkgo biloba flavonoids extraction efficiency, Comparison of AB-8, D101 two kinds of macroporous resin on the adsorption effect of Ginkgo flavonoids, obtain the extract of ginkgo biloba extract best technology and best purified resin。 Type with D101 macroporous resin on through the optimum extract of ginkgo biloba extract essence of purified products。 The experiment to get the best technology of extraction as follows: extracted by 50% ethanol as solvent, in the case of material liquid than 1:30 extract 5 hours 80 degrees Celsius。 Type with D101 macroporous resin purification vacuum concentration after freeze drying rate was 3%。

Key word: Ginkgo biloba, Flavonoids, Optimum process

目录

引言 1

1 材料与方法 3

1。1 供试材料 3

1。1。1实验材料 3

1。1。2实验仪器 3

1。1。3实验试剂 3

1。2实验方法 3

1。2。1试剂配制 3

1。2。2 供试材料的处理 3

1。2。3制作标准曲线 4

1。3 银杏黄酮提取工艺优化研究 4

1。3。1提取溶剂的研究 4

1。3。2提取料液比的研究 4

1。3。3提取时间的研究 5

1。3。4提取温度的研究 5

1。3。5计算总黄酮的提取率 5

1。4 银杏黄酮分离工艺优化研究 5

1。4。1 大孔树脂的预处理 5

2 结果与讨论 6

2。1 标准曲线 6

2。2最佳工艺的确定 6

2。2。1提取溶剂的研究 6

2。2。2提取料液比的研究 7

2。2。3提取时间的研究 8

2。2。4提取温度的研究