摘 要:为测定茶中酚量的最优提取工艺,分别以提取时间、提取温度、料液比3个单因素试验,考察各因素对总酚提取量的影响,以福林酚、没食子酸、无水碳酸钠为分析试剂,在单因素试验的基础上,以总酚含量为响应值,采用Box-Behnken试验设计的原理,对酚提取量进行三因素响应面实验。结果表明:确定维吾尔茶酚量最优提取工艺为:提取时间24min、提取温度85。5ºC、液料比1∶54(g/g),维吾尔保健茶总酚含量理论值为47。64mg/g。对最优的提取工艺可靠性进行检验得出酚提取量为46。8594mg/g。维吾尔茶的理论值和提取量十分接近,说明提取工艺有效。93567
毕业论文关 键 词:维吾尔茶酚量,提取时间,提取温度,料液比
Abstract: In order to determine the optimal extraction technology of the phenol in tea, with extraction time, extraction temperature, material liquid than 3 single factor experiment, studying the effect of various factors on the amount of total phenolic extraction, with the forint phenol, gallic acid, anhydrous sodium carbonate as analytical reagent, on the basis of single factor experiment, the total phenol content as the response value, the Box - Behnken the principle of design of experiment, three factors response surface experiments on phenol experiment。 Results show that the optimal extraction technology for determine the amount of uygur tea polyphenols: of 24 min time, extraction temperature 85。5 ℃, liquid ratio 1:54 (g/g), uygur health care tea total phenol content in the theoretical value of 47。64 mg/g。Based on the conclusion from the optimal extraction process of reliability test of phenol extraction quantity is 46。8594 mg/g。Uighur tea and extraction amount is very close to the theoretical value, extraction process effectively。
Key words: The quantity of uygur tea phenol, extracting time, extracting temperature, liquid ratio
目录
1。1 维吾尔茶 5
1。1。1 维吾尔茶的文化 5
1。1。2 维吾尔茶的种类 5
1。2 茶多酚 5
1。2。1 茶多酚的简介 5
1。2。2 茶多酚的成分 6
2 材料与方法 6
2。1 配方材料 6
2。2 实验试剂 6
2。3 仪器与设备 6
2。4 试验方法 7
2。4。1。 标准曲线的制作 7
2。4。2。 维吾尔保健茶总酚含量的测定 7
2。4。3 维吾尔保健茶总酚提取的单因素试验 7
2。4。4 响应面分析法优化工艺条件 8
2。5 抗氧化能力测定 8
2。5。1 配制不同稀释梯度的维吾尔保健茶提取液 8
2。5。2 数据处理 8
3 结果与讨论 8
3。1单因素试验结果 8
3。2响应面试验结果 10
3。2。1 BOX-BEHNKEN试验设计及结果 11
3。3 响应面分析与工艺优化 11
结论