摘要：百香果多酚是百香果中一种重要营养活性成分。本文采用响应面实验法优化超声协助乙醇提取百香果多酚的工艺条件，用大孔树脂技术分离纯化百香果多酚提取物，并对提取物进行抗氧化研究。研究结果表明，整果粉末在超声功率为450W、温度为80℃、乙醇浓度为30%及超声时间5min的条件下，百香果多酚的提取量达到12。49mg/g；果皮粉末在超声功率为450W、温度为70℃、乙醇浓度为80%和超声时间34min条件下，总多酚提取量达到12。5mg/g。HP-20树脂是一种快速吸附性树脂，并对百香果多酚表现出较好的吸附性能和解吸效果，吸附率为66。11%，解吸率为92。84%。此外，在抗氧化研究中，百香果多酚对DPPH自由基清除率最高分别为果皮78。01% 和整果80。94%，百香果多酚对ABTS+自由基清除率最高分别为果皮51。82% 和整果89。34%，证明百香果多酚是一种天然有效的抗氧化剂，且除果皮外其他组成部分中均含有多酚物质。74961

毕业论文关键词  百香果多酚 超声波提取 响应面试验 大孔树脂 抗氧化活性

Optimization of Extraction of Polyphenol from Passion Fruit by Response Surface Methodology and Evaluation of Antioxidant Activities

Abstract: Passion fruit Polyphenol is an important nutrient active ingredient in passion fruit。 In this paper, the reaction surface experiment was used to optimize the extraction conditions of ethanol extract of passion fruit by ethanol。 The macroporous resin was used to separate and purify the extract of passion fruit, and the antioxidant was studied。 The results showed that the extraction capacity of the fruit powder was 12。49mg / g under the conditions of ultrasonic power of 450W, temperature of 80 ℃, ethanol concentration of 30% and ultrasonic time of 5min。 The ultrasonic powder was 450W, the temperature was 70 ℃, the ethanol concentration was 80% and the ultrasonic time was 34min, the total polyphenol extraction was 12。5mg / g。 HP-20 resin is a kind of fast adsorbable resin, and it has good adsorption and desorption effect on the polyphenols of passion fruit。 The adsorption rate is 66。11% and the desorption rate is 92。84%。 In addition, the highest DPPH free radical scavenging rate was 97。01% and 80。94%, respectively。 In the antioxidant study, the highest removal rate of ABTS + free radicals was 51。82% and 89。34% %, To prove that passion fruit polyphenols is a natural and effective antioxidants, and in addition to other parts of the skin contains polyphenols。

Key words: Passion fruit polyphenol; Ultrasonic extraction; Response Surface Methodology; Macroporous resin; Antioxidant activity

目录

1 前言 1

1。1多酚研究现状 1

1。2百香果研究概述 1

  1。2。1百香果介绍 1

  1。2。2百香果研究现状 3

  1。2。3百香果的药理作用 3

1。3提取纯化技术 4

  1。3。1超声波提取法 4

  1。3。2微波提取法 4

  1。3。3超临界流体萃取法 4

  1。3。4连续回流提取法 5

1。4大孔树脂吸附提纯概述 6

1。5响应面方法（RSM）回归分析及BBD（Box-Behnken Design） 7

1。6本课题的目的及研究意义 7

2 材料与方法