摘 要：背景 酸浆是一种药食两用传统中药受到国内外学者的关注。目的 探讨酸浆根、茎、叶、宿萼和浆果醇提物的抑菌、抗氧化和抗肿瘤性。 方法 采用牛津杯法检测酸浆五种部位醇提物对金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌的抑菌活性；DPPH清除法检测其抗氧化活性；Alamar Blue法检测其对人乳腺癌细胞株MCF-7、MDA-MB-231和肝癌细胞株Bel7402的抑制效果。结果：浆果对金黄色葡萄球菌和大肠杆菌的抑菌效果最好，叶对枯草芽胞杆菌抑菌活性较明显。宿萼的抗氧化活性最高，浓度为5 mg/mL时对DPPH的清除率为97。87%，基本和同剂量的Vc效果相当。酸浆各部位醇提物对肿瘤细胞的抑制作用呈现浓度依赖性，其中宿萼的抗肿瘤细胞活性较明显。 结论 酸浆具有一定的抑菌、抗氧化、抗肿瘤，为酸浆的功能开发及活性成分分离提供一定的理论依据。79905

毕业论文关键词：酸浆、抑菌活性、抗氧化活性、抗肿瘤活性

Comparison study on activities of the ethanol extract from five parts of Physali alkekengi L。

Abstract: Background Physali alkekengi L。, as a traditional Chinese medicine, was paid attention by researchers all over the world。 Objective: The antimicrobial, antioxidant and anticancer activities of the different parts of Physali alkekengi L。 were evaluated in this study。 Methods: The antimicrobial activities against Staphylococcus aureus、Escherichia coli and Bacillus subtilis of the alcohol extracts of different parts were investigated using Oxford cup method。 The antioxidant activities were evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay。 The inhibition effects of human cancer cell lines MCF-7, MDA-MB-231 and Bel7402 were detected by Alamar Blue assay。 Results The alcohol extract of fruit showed the highest antimicrobial activity against S。 aureus and E。 coli。 Besides, the alcohol extract of leaves showed the highest antimicrobial activity against B。 subtilis。 The alcohol extract of calyx exhibited 97。87% DPPH radical scavenging efficiency at the concentration of 5 mg/mL, which demonstrated the similar antioxidant activity with the same dose of Vc。 All alcohol extracts of five parts showed inhibition activities on human cancer cells in a dose-dependent manner，furthermore, the alcohol extracts of calyx showed better inhibition activities。 Conclusion P。alkekengi L。 has certain antibacterial, antioxidant and anti-tumor activity。 These results provided theoretical basis for the functional development, further utilization of P。 alkekengi L。

Key words: Physalis alkekengi L。; antibacterial activity; antioxidant activity; anti-tumor activity

目 录

引言 1

1。实验材料与方法 2

1。1材料 2

1。1。1供材材料 2

1。1。2实验试剂 2

1。1。3实验器材 2

1。2方法 2

1。2。1 酸浆各部位样品提取 3

1。2。2抑制病原细菌实验 3

1。2。3 抗氧化活性实验 3

1。2。4 细胞培养与体外抗肿瘤细胞活性实验 4

1。2。5 统计分析 4

2。实验结果与分析 5

2。1酸浆各提取物抑菌活性的对比分析 5

2。2酸浆各提取物的抗氧化活性 6

2。3酸浆各提取物对肿瘤细胞的影响