FLS900大蓟与小蓟荧光光谱特性对比研究
毕业论文关键字：荧光光谱 偏振 发射 精确辨别
Thistle and field thistle fluorescence spectral characteristics contrast research
Abstract: The FLS900 type fluorescence spectrometer was used to study the thistle and field thistle fluorescence spectrum. Through the experiment measured the thistle and field thistle excitation spectrum, and thistle under different concentrations and field thistle emission spectrum and polarization spectrum, and by use of OriginPro software, draw the spectral curve. Analysis found spectral curve, thistle excitation peak is located at 408 nm, field thistle excitation peak is located at 429 nm. Contrast thistle and field thistle excitation spectra and emission spectra, under the same concentration, the excitation, emission peak position, peak and peak strength is different; In different concentrations, thistle emission peak at 475 nm to 477 nm, the field thistle emission peak at 510 nm to 512 nm; The experimental results show that the thistle and field thistle on spectral differences is large enough, can accurately identify thistle and field thistle by using fluorescence spectroscopy.
Key words: fluorescence spectrum polarization excitation accurately distinguish
目录
1引言 1
1.1中药材鉴别 1
1.2研究对象 3
1.2.1大蓟 2
1.2.2小蓟 2
1.3分子荧光的产生 3
1.4荧光的激发、发射光谱 4
1.4.1荧光激发光谱 4
1.4.2荧光发射光谱 4
1.4.3荧光激发、发射光谱的联系 4
1.5荧光光谱的主要参量 4
1.5.1荧光强度 4
1.5.2荧光偏振度 4
2实验 5
2.1实验装置 5
2.2样品制备 6
2.3实验操作 6
3实验结果与分析 8
3.1大蓟荧光光谱特性研究 8
3.1.1大蓟激发光谱与激发波长 9
3.1.2大蓟溶液不同浓度下的发射光谱 10
3.1.3大蓟溶液在不同浓度下的偏振光谱 11
3.2小蓟荧光光谱特性研究 11
3.2.1小蓟的激发光谱与激发波长 11
3.2.2小蓟溶液在不同浓度下的发射光谱 12
3.2.3小蓟溶液在不同浓度下的偏振光谱 13
3.3激发光谱对比研究 15
3.4相同浓度下发射光谱对比研究 16
4结论与展望 17
4.1结论 17
4.2展望 17
致谢 19
参考文献 20
1引言
在中医药术指导下应用的药物就是指中药（Chinese herbology、Traditional Materia Medica）。是由许许多多的中医在实践过程中一点一滴的积累起来的。有中成药、中药材两个类别。中药主要是由中国发起的，其中主要包括植物药、动物药、矿物类、介壳类等，只有少部分的中药来源于外国。因此中药在我国历史悠久，是全国人民的共同拥有的一份宝贵财富。中药的用途也十分广泛，在人们心中的可信度也在逐步提高。中药讲究从根本上去预防和治疗疾病，虽然见效速度比西药慢，但以其副作用较小，疗效佳，成为医生开药时的首选药。
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