摘    要   经实验表明当处于压力下时，人和啮齿动物会增加可口的食物的消耗，这类高卡路里高热量食物具有减轻压力的潜在性质。最新实验研究也发现高卡路里高热量食物通过奖赏通路影响和改善小鼠的焦虑行为。而终纹床核（Bed nucleus of stria-terminalis,BNST）在应激和奖赏通路中都具有重要的作用,应激会使BNST中促肾上腺皮质激素释放激素 CRF(Corticotropin releasing factor) mRNA的表达增加,并且CRF mRNA的过表达是焦虑产生的重要机制。89589

实验以雄性SD大鼠作为研究对象,采用减压舱进行低氧暴露,通过行为观察的方法,比较研究低氧暴露前和低氧暴露期间糖摄入对大鼠焦虑行为的影响, 以及BNST中CRF mRNA的表达量，探讨低氧暴露与糖摄入对焦虑行为的耦合作用。

实验将大鼠分为对照组（C），5000米低氧组（H），喂食蔗糖不低氧应激组（SC），喂食蔗糖且低氧应激组（SH）。大鼠的饲养严格遵照实验室大鼠的饲养方法,温度22±1℃,湿度60±10%,光照周期12L:12D,饲料、饮用水充足。所有实验中的大鼠每日在固定时间进行体重、摄食量的称量,低氧暴露1天后对大鼠进行焦虑行为的检测。

实验结果：

1、低氧暴露一天后，SH组相较于SC组，蔗糖摄取量显著增加。

2、低氧暴露前和低氧暴露中糖摄入都能明显缓解焦虑行为，但分别在站立次数、危险情境停留时长上与低氧组无显著性差异。

3、低氧暴露可导致BNST中的CRF mRNA显著表达；蔗糖摄入缓解了低氧暴露后BNST中的CRF mRNA表达的增加

由以上结果表明：低氧暴露诱导大鼠产生焦虑行为，而这种焦虑行为的产生可能是BNST中CRF mRNA表达增加所致，而糖摄入可能通过减少低氧暴露后的BNST中CRF mRNA表达的增加从而缓解低氧暴露引起的焦虑行为。

毕业论文关键词： 低氧;  应激； 糖摄入； 焦虑行为

Abstract Experiments show that people and rodents increase the consumption of delicious food when under pressure, and such high calorie high calorie foods have the potential to reduce stress。 The latest experimental study also found that high calorie high-calorie foods affect the anxiety behavior of mice through reward pathways。 (BNST) plays an important role in stress and reward pathways, and stress increases the expression of corticotropin releasing factor (CRF) in BNST , And overexpression of CRF mRNA is an important mechanism of anxiety。 

In this study, male SD rats were used as the research object。 Hypoxia exposure was performed in decompression chamber。 The effects of glucose intake on anxiety behavior in rats before and after hypoxia exposure were studied by behavioral observation。 CRF mRNA expression, and explore the coupling effect of hypoxia exposure and sugar intake on anxiety behavior。

Rats were pided into control group (C), 5000 m hypoxia group (H), fed with sucrose without hypoxia stress (SC), fed with sucrose and hypoxia stress group (SH)。 Rats were housed inpidually and maintained on a 12:12-hr light-dark cycle (lights on at 7 a。m。), 22±1℃ temperature, 60±10% humidity, free to feed and drink water。 All experimental rats to weighing weight and food intake in provision time。 The rats which in hypoxia exposure 1 day group did the test for anxiety。源Q于W优E尔A论S文R网wwW.yOueRw.com 原文+QQ75201,8766

Experimental results:

1, after a week of hypoxia exposure, SH group compared with the SC group, sucrose intake increased significantly。

2, before and after hypoxia exposure, glucose intake can significantly relieve anxiety, but there was no significant difference between the number of standing and the duration of risk in the hypoxia group。

3, hypoxia exposure can lead to significant expression of CRF mRNA in BNST; sucrose intake alleviated the increase in CRF mRNA expression in BNST after hypoxia exposure