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    bacteria in the room before the cleaning gradually reduced over time; then it has
    the highest number of bacteria after getting up; then as no one cleaning, thenumber of bacteria in two different rooms will be reduced; bacteria begin to rise
    after cleaning to the second detection of bacteria exceeded a multiple of the
    maximum and then decreased. I believe that is because the cleaning makes the
    increase in the number of airborne bacteria, bacterial super-multiples of the room
    will be a sharp decline until the time of the cleaned up after some time.
    By comparison, the air-conditioned rooms’ bacteria number is always higher
    than naturally ventilated rooms, so to enhance natural ventilation is to reduce the
    total number of indoor bacteria, and cleaning is the main cause of secondary
    pollution, but this cannot conclude the air-conditioned room air quality is poorer
    than naturally ventilated rooms. There is the need for further analysis.
    52.4.2 Deep Inspection
    Although the number of bacteria obtained from the macroscopic response be
    processed there and air quality, but to draw the exact results, but also on the
    bacterial composition further research and identification, analysis of bacterial
    components.Analysis through the study of bacteria, Gram-positive bacteria accounted for
    most (about 78 %); composition of the fungi is shown in Table 52.3.
    Fungal ingredients are mainly spores, and the percentage of the various com-
    ponents are drawn in curves and shown in Fig. 52.3.
    From Table 52.3 and Fig. 52.3, we can analyze whether it is air-conditioned
    rooms or natural ventilation rooms, various types of fungi in the proportion
    remained at a relatively balanced level, and spores percentage is relatively higher
    in the air-conditioned rooms; other types of fungi is relatively higher in the natural
    ventilation conditions.
    52.5 Conclusion
    Based on the above findings and through comparative study of air quality of the
    natural ventilation rooms and air-conditioned rooms in the hotel, we know that the
    bacterial number of air-conditioned room is more than the naturally ventilated
    rooms, and the bacterial composition in terms of overall similarity, proportion of
    various types of fungi is stable, but the proportion of spore is higher in the natural
    ventilation room, but the air quality under the two conditions are able to meet the
    normal hotel use. Through comparative analysis of the data, we can clearly see that
    the air quality in the hotel room is good or bad is not just related to air conditioning
    and natural ventilation, but also to human factors, and the impact on air qualityfactors are very complex, which must be considered in order to make hotel rooms
    have the best living environment. Hotel rooms are designed to comprehensively
    consider the local climatic conditions, surrounding environment, and among other
    factors, to achieve the organic combination of natural ventilation and air condi-
    tioning systems. It is necessary to consider the space ventilation advantages and
    the good effects of natural ventilation so as to create a comfortable environment
    for both customers and the staff.
    References
    1. Mike Harg IL (1992) Design with nature, vol 32. China Building Industry Press. Rui Jingwei
    translation, Beijing, pp 55–78
    2. Xue DH (1991) Air-conditioning, vol 87. Tsinghua University Press, Beijing, p 110
    3. Liu J, Wu YW, Wang QS (2000) Discussion on indoor air quality, ventilation and air
    conditioning problems. Chin Health Eng 12(6):87–99
    4. Zhao RM, Fu ZH, Liang QC, Chen LU (2011) Survey of health conditions of public places air
    conditioning system in Haikou city. Chin Trop Med 5(12):76–88
    5. Shen JM (1998) Indoor air quality assessment. HVAC 50(4):225
    6. Zhang ZY, Shang ML (1997) Relationship between the total number of bacteria in the air and
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