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摘要： 细菌纤文素(Bacterial Cellulose,BC)是一种新型生物材料，其独特的组织结构, 高吸水性，高保水性、高旋光性以及高透过率，纯度高的性能使细菌纤文素用在各种方面。本课题通过在发酵培养基内添加聚丙烯酰胺和聚乙烯醇，利用木醋杆菌静态发酵细菌纤文素/聚丙烯酰胺/聚乙烯醇复合膜，并测定复合膜的含水量、复水率、红外光谱和膜拉伸性能。结果表明：PAM与PVA添加比例为3:30时，复合膜的产量最高，含水量最高可达到98.63%，复水率最高97.24%。PAM与PVA的比例为时1:10时，拉伸强度和断裂伸长率均为最大，分别为0.282MPa和53.24%.24236
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毕业论文关键词：木醋杆菌；细菌纤文素；细菌纤文素/聚丙烯酰胺/聚乙烯醇复合膜；表征
Preparation and Characterization of Bacterial Cellulose / Polyacrylamide / PVA Composite Membrane
Abstract: Bacterial cellulose(BC) is the new biological material. Its unique structure, high water absorption, high water retention, high optical properties and high transmittance and high purity make bacterial cellulose used in various aspects. This paper prepare bacterial cellulose /Polyacrylamide / PVA composite membrane by adding PAM and PVA into bacterial cellulose fermentation medium ,then determined water content, rehydration ratio, infrared spectroscopy and tensile properties of the membrane. The results showed that when the proportion of PAM and PVA was 3:30，the yield of composite film was the highest, its water content of up to 98.63%, the rehydration rate up to 97.24%．when the proportion of PAM and PVA was 1:10,the tensile strength and elongation were the highest 0.282MPa and 53.24% respectively.
Key words: Acetobacter xylinum; Bacterial Cellulose; Bacterial Cellulose /Polyacrylamide/ Polyvinyl alcohol composite membrane;Characterization
目录
1 绪论 1
1.1 细菌纤文素简介 1
1.2 细菌纤文素的国内外研究现状 1
1.2.1 细菌纤文素的结构 2
1.2.2 细菌纤文素的性质 3
1.2.3 细菌纤文素的应用 3
1.3 聚丙烯酰胺和聚乙烯醇简介 5
1.3.1 聚丙烯酰胺和聚乙烯醇的理化性质 5
1.3.2 聚丙烯酰胺和聚乙烯醇的应用 6
1.4 细菌纤文素复合材料 7
1.5 研究的目的和意义 7
2 材料和方法 8
2.1 材料 8
2.1.1 实验菌种 8
2.1.2 实验试剂 9
2.1.3实验设备 9
2.2 实验方法 10
2.2.1 溶液配制 10
2.2.2 木醋杆菌种子液制备 10
2.2.3 细菌纤文素膜的制备与处理 10
2.3 细菌纤文素复合膜持水性测定实验 11
2.3.1 细菌纤文素复合膜含水量的测定 11
2.3.2细菌纤文素复合膜复水率测定 11
2.4 细菌纤文素复合膜红外光谱测定实验 11
2.4.1 测定方法 11
2.5 细菌纤文素复合膜拉力的测定 11
2.6 细菌纤文素复合膜形态观察 12
2.6.1 细菌纤文素复合膜湿膜的形态 12
2.6.2 细菌纤文素复合膜干膜的形态 12
2.6.3 细菌纤文素复合膜冷冻干燥膜的形态 12
3 结果与分析 12
3.1细菌纤文素复合膜含水量和复水率的测定 12
3.2 冷冻干燥的细菌纤文素复合膜膜重的测定 13
3.3 细菌纤文素复合膜红外光谱测定 15
3.4 细菌纤文素复合膜的拉伸强度和断裂伸长率的测定 16