摘要：本研究优化了榆黄多糖的提取工艺，并对榆黄提取液化妆品进行制备和功效评价。通过单因素实验和相应面实验获得榆黄多糖最优提取条件为：料液比为1:41，提取温度为90℃，提取时间为42min。在此最优条件下得到的榆黄子实体提取液经浓缩至1g/mL后，分析浓缩液中各功效成分的含量，分别为：多肽为27.91mg/mL，黄酮为1.32mg/mL，总酚为1.74mg/mL，总氨基酸为27.64mg/mL，总蛋白为88.93mg/mL，麦角硫因5.18mg/g，多糖66.80mg/mL。所得榆黄提取物具有羟自由基清除能力、还原力、过氧化氢清除能力等抗氧化功能。所得化妆品具备较好的保湿性能和感官评价及稳定性。

关键词：榆黄；提取优化；抗氧化；成分分析；化妆品

Preparation and efficacy evaluation of a cosmetic product containing pleurotus citrinopileatus

Abstract ：This study has optimized the extraction process of the polysaccharides from Pleurotus citrinopileatus and the preparation and efficacy evaluation of the Pleurotus citrinopileatus extract cosmetics. The optimum extraction conditions for the polysaccharides were obtained by single factor experiments and corresponding surface experiments: the ratio of material to liquid was 1:41, the extraction temperature was 90°C, and the extraction time was 42 min. Under the optimal conditions, the extract of Pleurotus citrinopileatus fruiting body was concentrated to 1g/mL, and the content of each functional component in the concentrated solution was analyzed as follows: polypeptide was 27.91 mg/mL, flavonoid was 1.32 mg/mL, total The phenol was 1.74 mg/mL, the total amino acid was 27.64 mg/mL, the total protein was 88.93 mg/mL, ergotthione 5.18 mg/g, and the polysaccharide 66.80 mg/mL. The obtained Pleurotus citrinopileatus extract has antioxidant functions such as hydroxyl radical scavenging ability, reducing power, and hydrogen peroxide scavenging ability. The resulting cosmetics have good moisturizing properties and sensory evaluation and stability.

KeyWords: pleurotus citrinopileatus;Extraction optimization;; Composition analysis; Cosmetics;Antioxidant

目 录

1绪论 1

1.1天然化妆品研究进展 1

1.2食用菌在化妆品中的应用 3

1.3榆黄菇的开发与应用研究进展 4

1.4意义与研究内容 6

2材料与方法 7

2.1实验原料 7

2.2实验方法 7

2.2.1榆黄菇提取条件优化 7

2.2.2榆黄菇提取液功效成分含量的测定 8

2.2.3榆黄菇提取液的抗氧化性评价 12

2.2.4体外酪氨酸酶抑制能力方法 12

2.3榆黄菇提取液化妆品中的制备 12

2.3.1基础配方 12

2.3.2制备工艺 14

2.3.3保湿功效测定方法 14

2.3.4感官评价及理化指标 16

3结果讨论 18

3.1榆黄提取条件优化 18

3.1.1单因素实验 18

3.1.2响应面实验 19

3.2榆黄提取液功效成分 22

3.2.1成分分析