链格孢内生菌株对加拿大一枝黄花植物的化感作用研究
毕业论文关键词：植物内生菌；化感作用；种子；发芽率。
Abstract In this paper, the secondary metabolites of an endophyte F4 isolated from the leaves of Solidago canadensis were collected and the allelopathic effect of them on five kinds of plant seeds , Suzhou green, cucumber, radish and black sesame seeds, was also studied。 The results showed that the ethyl acetate extract of F4 strain fermented broth was most pronounced effect on rapeseed and black sesame。 Separated by the methods of silica gel column chromatography and thin layer chromatography detection, we obtained three organic solvent elution segments from the F4 fermentation extract。 The effects of three segments on rape and black sesame seeds were different at the concentration of 0。5mg / ml and 1mg / ml。 With increasing concentration of different elution segments, the inhibition on seed germination and seedling growth of the two plant increased。 But low concentrations experimental groups could increase the weight of black sesame seedlings by promoting the formation of protein and polysaccharide substances, at the same time, also promoted the formation of chlorophyll a and b。 The low polarity elution segments showed more stronger inhibition on rape seed germination and seedling growth。 The weight of seedling was suppressed by decreasing the production of protein and polysaccharide。 With the increase of concentration, the inhibition of the metabolite for two kinds of seed were stronger。
Key words: Endophytic Fungus; Allelopathy。 Seeds; Germination Rate
目录
第一章 绪论 1
1。1 前言 1
1。2 植物内生真菌存在的普遍性与多样性 1
1。3 植物内生菌的抗虫作用 2
1。3。1 内生菌提高宿主的抗虫性 2
1。3。2植物内生菌代谢物对害虫的抗性 2
1。3。3 内生菌抗虫活性的主要机制 2
1。4 植物内生菌对植物的作用 3
1。5微生物之间的化感作用 4
1。6 植物内生真菌的化感物质 4
1。6。1 酚类化感物质 4
1。6。2 萜类化感物质 4
1。6。3生物碱类化合物 5
1。7实验目的及意义 5
1。8研究内容及技术路线 6
1。8。1 研究内容 6
1。8。2 技术路线 6
第二章 材料与方法 7
2。1 实验材料 7
2。1。1 菌株和受体植物来源 7
2。1。2 实验试剂与器材 7
2。1。3 培养基 8
2。1。4 主要试剂的配置 8
2。2 实验方法 8
2。2。1菌株的活化 8
2。2。2 菌株的发酵培养 8
2。2。3 发酵产物浸膏和菌体浸膏的制备 9