低剂量农药胁迫下绿萝可溶性糖游离氨基酸总量及其根活力变化
毕业论文关键词： 百菌清；吡蚜酮；根系活力；绿萝；Hormesis效应
Changes of the content of soluble sugar and amino acid and the activity of the root in Scindapsus aureus under the stress of low-dose pesticide
Abstract: As an effective means of controlling harmful organisms, chemical pesticide plays a very important role in plant training. But impact on plants in the low-dose dose did not receive enough attention. Changes of the content of soluble sugar and amino acid and the activity of the root in Scindapsus aureus under the stress of low-dose pesticide were studied in this paper. The results from the research of pesticide to the root activity showed that the root in activity S. aureus was stimulated by the 8, 64ppm of chlorothalonil. And the activity of root in S. aureus was increased significantly after the treatment of pymetrozine. The content of the amino acid was decreased significantly after the treatment of pymetrozine, and the content of the soluble sugar was increased significantly. However, the content of the soluble sugar in S. aureus was increased significantly at the treatment of 8,16,32,64ppm chlorothalonil. And with the increasing of the concentration of chlorothalonil, the content of the amino acid showed an inverted “U” type feature, which is the typical characteristics of the hormetic effect. The result showed that the hormesis of the amino acid in S. aureus was induced by the low-dose chlorothalonil.
Key words: chlorothalonil; pymetrozine; the activity of root; S. aureus; Hormatic effect
目录
1 绪论 1
1.1 农药对植物影响研究 1
1.1.1 农药对作物主要营养物质的影响 1
1.1.2 农药对作物根活力的影响 2
1.1.3 逆境条件对植物可溶性糖含量、游离氨基酸含量以及根活力的影响 2
1.2绿萝 3
1.3 Hormesis效应 3
1.4 研究低剂量农药胁迫下绿萝可溶性糖、游离氨基酸总量及其根活力变化的目的及意义 4
1.4.1 目的 4
1.4.2 意义 4
1.5 本课题应解决的主要问题 4
2 材料与方法 5
2.1 材料 5
2.1.1绿萝 5
2.1.2绿萝培养 5
2.2 农药和应用 5
2.2.1 农药 5
2.2.2 农药配制 6
2.3 绿萝植株中的可溶性糖含量测定 6
2.3.1 试剂的配置 6
2.3.2 实验步骤 6
2.4 绿萝植株中的氨基酸含量测定 7
2.4.1 试剂的配制 7
2.4.2 实验步骤 7
2.5 绿萝植株的根活力测定 8
2.5.1 试剂的配置 8
2.5.2 实验步骤 8
2.6 统计分析 9
3 结果与分析 9
3.1 两种农药对绿萝可溶性糖含量影响研究 9
3.2 两种农药对绿萝游离氨基酸总量的影响研究 10
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