低剂量抗生素土霉素对水生生物蓝藻的毒性研究
毕业论文关键词： 土霉素；蓝藻；毒性；抑制
Toxicity of low-dose Oxytetracycline on Cyanobacteria
Abstract: For a long time, antibiotics are used in the treatment of disease of human and animals. Among them, tetracycline antibiotics (such as oxytetracycline and tetracycline etc.) are used most in the world. Both antibiotics and cyanobacteria causing water pollution are important issues of today's water environment. They are generally present in the water. However, the study on release of oxytetracycline toxicity towards algal is very lack, therefore this study will not only help us to understand the characteristics of oxytetracycline and cyanobacteria further but also aim to further understand and master the oxytetracycline chemical properties and biological effectiveness. This paper introduces the tetracycline antibiotics pollution and ecological toxicity by taking the cyanobacteria as the research object. Through the inhibitory effect on the growth of cyanobacteria and the effect on protein content, we will understand the toxicity of oxytetracycline on cyanobacteria.
KeyWords: Oxytetracycline; Cyanobacteria; toxicity; Inhibit
目录
1 引言 1
1.1 抗生素的污染现状 1
1.2 土霉素的污染现状 2
1.3 四环素类抗生素（土霉素）的生态毒性 3
1.3.1 四环素类抗生素（土霉素）对微生物的生态毒性 3
1.3.2 四环素类抗生素（土霉素）对植物的生态毒性 4
1.3.3 四环素类抗生素（土霉素）对动物的生态毒性 4
1.4 土霉素的理化性质和药理毒理 5
1.4.1 土霉素的理化性质 5
1.4.2 土霉素的药理毒理 6
1.5 蓝藻 6
1.5.1 蓝藻概述 6
1.5.2 蓝藻特性 6
1.5.3 藻类水华与水体富营养 7
1.6 课题研究的意义和目的 7
1.7 课题研究的内容 7
2. 实验条件 8
2.1 实验仪器 8
2.2 实验材料 10
2.3 实验药品 10
3 实验内容 10
3.1 蓝藻的培养 10
3.1.1 BG11培养液的配置 10
3.1.2 高压蒸汽灭菌 11
3.1.3 蓝藻的接种 11
3.1.4 蓝藻的培养 11
3.2 实验方案论证 12
4 实验方案与方法 12
4.1 土霉素储备液的配置 12
4.2 蓝藻生长曲线及抑制率 12
4.3 考马斯亮蓝染色法测定蛋白质含量 13
4.3.1 实验原理 13
4.3.2 试剂配制 13
4.3.3 实验方法 13
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