蓝光连续光照对大豆芽苗菜花青苷合成的影响
毕业论文关键词：大豆芽苗菜；花青苷；蓝光
Effect of blue light continuous illumination on anthocyanin Synthesis in soybean sprouts
Abstract：In this study, ‘Dongnong 690’soybean sprouts were tested to investigate the effects of blue light continuous illumination on the growth and anthocyanin synthesis of soybean sprouts.Dark culture was used as control.The results showed that compared with the dark,under blue light treatment, the hypocotyl length of soybean sprouts was significantly inhibited, the edible rate was significantly reduced, and the root length was significantly increased.With the prolongation of light time, the content of anthocyanin in hypocotyls of soybean sprouts was gradually accumulated.The activity of phenylalanine ammonia lyase (PAL) and chalcone isomerase (CHI) and the expression of PAL, CHS and ANS in the hypocotyls of soybean sprouts in the treatment group were increased after the lower.Compared with dark culture, the expression of CRY1 and CRY2 were significantly increased under blue light continuous illumination.
Key words: soybean sprouts；anthocyanin；blue light
目 录
摘要1
关键词1
Abstract1
Key words1
引言（或绪论）1
1材料与方法2
1.1试验材料与方法 2
1.2测定项目与测定方法2
1.2.1生长指标的测定2
1.2.2花青苷含量的测定3
1.2.3花青苷合成过程中关键酶（PAL、CHI）活性的测定3
1.2.4总RNA的提取及荧光实时定量PCR分析3
1.3数据分析4
2结果与分析4
2.1蓝光连续光照对大豆芽苗菜生长的影响4
2.2蓝光连续光照对大豆芽苗菜下胚轴花青苷积累的影响5
2.3蓝光处理对大豆芽苗菜中花青苷合成相关酶（PAL、CHI）活性的影响5
2.4蓝光处理对蓝光受体及花青苷合成相关基因表达的影响6
3讨论 8
3.1蓝光连续光照对大豆芽苗菜生长的影响8
3.2蓝光连续光照对大豆芽苗菜下胚轴花青苷积累的影响8
3.3蓝光处理对大豆芽苗菜中花青苷合成相关酶（PAL、CHI）活性的影响8
3.4蓝光处理对蓝光受体及花青苷合成相关基因表达的影响8
致谢9
参考文献10
蓝光连续光照对大豆芽苗菜花青苷合成的影响
大豆富含蛋白质、氨基酸、不饱和脂肪酸、大豆异黄酮等多种营养物质，是我国重要的粮油作物[1]。研究发现，经发芽处理，大豆芽苗菜的营养物质及保健性功能成分显著高于发芽前[2]。大豆芽苗菜具有补气养血，防止心血管硬化，防治老年高血压等多种功效[3]，是一类对人体健康有益的常见食品。花青苷是黄酮类化合物中的一种，属于水溶性天然色素，同时具有强效的自由基清除功能，因而具有很强的抗氧化性，具有抗氧化衰老、抗癌、抗突变、降血脂等多种功效，在食品、医疗保健品等诸多方面都有重要研究价值[4]。因此，如何提高花青苷的含量日益受到科学界的重视。
有研究表明，蓝光能增加野生型拟南芥及番茄中的花青苷含量[5-6]。在植物栽培过程中如果采用连续光照的方法，即使用人造光保持光强与光质恒定不变，使光照时间超过24 h，可以提高产量与品质[7-8]。
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