棉铃虫种群Bt抗性检测及显隐性分析
毕业论文关键词：棉铃虫；Bt毒素；抗性检测；显隐性分析
Detection and genetic analysis of Bt resistance in cotton bollworm from Gaoyang in 2016
Abstract: The insecticidal protein produced by Bacillus thuringiensis (Bt) can effectively kill many major insect pests, it is widely used in the field of biological insecticide and transgenic Bt gene crops. Transgenic cotton producing Bt toxin Cry1Ac (Bt cotton) has been widely cultivated in China for 20 years. It is regarded as inevitable that resistance to Cry1Ac of H. armigera will increase under intensive selection of Bt cotton in China. Therefore, it is very important to monitor the development of Bt resistance in the field and assess the resistance risk. This paper has detected the frequency of resistant inpiduals and the dominant ratio of resistance to Cry1Ac in Gaoyang area. It was found that the development of the resistance of cotton bollworm to Bt toxin in this area was rapid in recent years, and the frequency of resistant inpiduals in 2016 reached 16.8 %. One resistance strain to Cry1Ac was isolated and its resistance was completely dominant.
Key words: Helicoverpa armigera; Bt toxin; Detection of resistance; Genetic analysis
目 录
摘要1
关键词1
Abstract1
Key words1
引言（或绪论）1
1材料与方法2
1.1供试昆虫 2
1.2 Bt毒素 2
1.3 分离品系2
1.4高通量筛选法3
1.5单对测交实验3
1.6生物测定4
1.7数据处理4
2结果与分析4
2.1 2010-2016年间高阳县棉铃虫田间种群中抗性个体频率4
2.2抗性品系GY♀30的分离纯化4
2.3抗性品系GY♀30的抗性遗传5
3讨论6
致谢6
参考文献7
图1 高阳县GY♀30品系的分离3
图2 2010-2016年高阳县棉铃虫田间种群抗性个体频率4
图3高阳县田间种群F1代单对生物测定结果5
图 4在诊断浓度（1μg Cry1Ac/cm2）下棉铃虫幼虫存活率6
表 1 GY♀30品系对Cry1Ac剂量反应5
河北高阳2016年棉铃虫种群Bt抗性检测及显隐性分析
棉花作为一种重要经济作物在我国广泛种植，产量大，用途广，其种植业是我国农业的支柱性产业，对国民经济的发展至关重要，同时其产量和品质直接关系到农业生产水平。上世纪九十年代棉铃虫在我国棉区严重发生，1992年黄河流域棉铃虫发生量是常年的20多倍，全国棉花总产量减少了1/3，大暴发造成巨额的经济损失，严重制约了棉花的生产[1]。苏云金芽孢杆菌（Bacillus thuringiensis，简称Bt）产生的杀虫蛋白能够杀灭许多重要靶标害虫（主要为鳞翅目、鞘翅目害虫），但对脊椎动物和多数非靶标生物体无害[2,3,4]，因此作为生物杀虫剂被广泛用于害虫防治。利用基因工程产生的转Bt毒素基因的玉米和棉花等植物带来许多好处。Bt作物的大规模应用取得了显著的经济效益和环境效益，包括害虫的抑制，杀虫剂的使用减少，天敌的保护，产量的增加，农民利润的提高等。其中，表达Cry1A蛋白的基因是使用最广泛也是最有效的基因。特别是能杀死鳞翅目幼虫的三种毒素：来自Bt玉米的Cry1Ab、来自Bt棉的Cry1Ac和在第二代抗虫玉米和抗虫棉花中的Cry2Ab[5]。我国自1997年开始商业化种植转Bt基因棉花，2015