不同浓度霉菌毒素对卵子毒性效果的检测
毕业论文关键词：霉菌毒素；生殖系统；卵母细胞
The detection of toxic effects of different mycotoxins on oocytes
Abstract：Mycotoxins are colorless, odorless, tasteless secondary metabolites produced by mold or fungi. In the breeding industry, the mycotoxins produced by mildewed feed and cereals have a great impact on the health of the livestock, including the impact on the quality of the oocytes and thus the reproductive system of the livestock, which has caused an immeasurable loss to the livestock breeding. Although studies have shown that mycotoxins have a negative impact on the health of livestock, but so far the toxicity of mycotoxins to eggs is not studied systematically, and the sensitivity to mycotoxins between species is no more than that. To this end, the subject will be pig and mouse oocytes as the object of study, through the in vitro culture process to add different species, and the same species of different concentrations of mycotoxins to study its toxic effects on the oocytes, and compared pigs and mice Oocytes between the sensitivity of mycotoxins, for the relevant industry to provide theoretical basis and technical support.
Key words：mycotoxin； reproductive system； oocyte
目 录
摘要1
关键词1
Abstract1
Key words 1
引言1
1实验材料3
1.1实验对象与试剂 3
1.2试剂的配制 3
1.3实验仪器与设备 4
2 实验方法4
2.1 实验流程4
2.2 实验步骤5
2.2.1毒素的配制5
2.2.2小鼠卵母细胞采集与毒性检测5
2.2.3猪卵母细胞成熟培养与毒性检测5
3 结果分析6
3.1毒素对小鼠卵母细胞成熟的影响6
3.2毒素对猪卵母细胞成熟的影响7
3.3对比猪和小鼠之间物种的差异性8
4 讨论9
致谢10
参考文献10
不同浓度霉菌毒素对卵子毒性效果的检测
引言
21世纪以来，由于我国畜牧业的快速发展，畜禽养殖逐渐由小型散养转变为规模化大型养殖，繁殖率是限制养殖成本的主要因素之一，因此，人们开始关注畜禽的生殖和它们所处环境的关系。近年来，随着科学技术的发展和相关研究的不断深入，人们已经逐渐认识到霉菌毒素给养殖业带来的严重危害。霉菌是可以生长出分枝菌丝的真菌统称，凡是在基质上生长成棉絮状、绒毛状或蜘蛛网状菌丝体的真菌都称之为“霉菌”。霉菌在自然界分布广范，种类繁多，目前约记载就有3500～10000种，而谷物中常见的霉菌大约有200种[1]。霉菌毒素（Mycotoxin）是指霉菌在其所感染的有机物中所产生的级代谢产物[2]。霉菌可生长在田间作物上，产生的毒素称为田间毒素；也可生长在加工、运输和储存等环节，分泌的毒素称为仓储毒素。饲料中常见且对人和动物危害相对严重的霉菌毒素主要有黄曲霉毒素、玉米赤霉烯酮、呕吐毒素、赭曲霉毒素、T-2毒素及伏马菌素等。其中，玉米赤霉烯酮 (ZEN)、黄曲霉毒素B1和脱氧雪腐镰刀菌烯醇(DON) 是猪饲料中存在最为广泛，危害最为严重的三种霉菌毒素。