土荆芥微卫星引物开发+文献综述
毕业论文关键词：土荆芥，SSR，引物开发，遗传多样性分析
Development of SSR primers for Chenopodium ambrosioides L.
Abstract:Chenopodium ambrosioides L. has been proved to be hyperaccumulator for heavy metal, and it has a potential value of soil restoration. At present, the genetic variation of the Chenopodium ambrosioides L is unclear, therefore it is of great significance to develop its genetics from the perspective of molecular biology. Simple sequence repeat (SSR) markers are a widely used type of genetic marker for this purpose. It can reveal the genetic variation of species among different populations In this study, we used the DNA of Chenopodium ambrosioides L to develop the suitable primers for the SSR markers. We designed 100 pairs of primers by using high-throughput sequencing. 76 pairs of primers were able to amplify products and 15 pairs of primers were polymorphic. After that, systematic analysis was conducted on its genetic persity, genetic relationship, the degree of differentiation, genetic structure of group population of experimental materials included 322 samples from 18 groups of Chenopodium ambrosioides L. The results of the study will provide theoretical basis for the invasion pathways of Chenopodium ambrosioides L. in China and the evolutionary mechanisms of heavy metal tolerance. Keywords:Chenopodium ambrosioides L., SSR, SSR Primer develop, Genetic persity analysis.
目 录
摘要1
关键词1
Abstract1
Key words 1
引言1
1.材料与方法2
1.1 材料的采集与处理2
1.2 土荆芥 DNA 的提取3
1.3 土荆芥 SSR 位点的鉴定与 SSR 引物的设计3
1.4 DNA质量的检测 4
1.5 SSR-PCR扩增 4
1.6 SSR-PCR产物检测 4
1.7 荧光毛细管电泳测序4
1.8 数据分析4
2.结果与分析5
2.1 土荆芥 SSR 引物筛选结果分析5
2.1.1 土荆芥 SSR 组成分析5
2.1.2 土荆芥 DNA 提取结果6
2.1.3 土荆芥 SSR 引物的筛选 7
2.2 土荆芥遗传多样性分析 8
2.2.1 土荆芥群体间遗传多样性分析 8
2.2.2 PCoA主成分分析 9
2.2.3 土荆芥群体遗传结构9
2.2.4 土荆芥居群聚类分析 10
2.2.5土荆芥种群间遗传分化11
3.讨论 11
致谢 13
参考文献 13
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