ABA、GAs和温度协同作用对芹菜种子萌发的影响
毕业论文关键词：芹菜；种子；赤霉素；脱落酸
Effects of ABA, GAs and temperature on the germination of Celery Seeds
Abstract： As one of the main vegetables grown in large areas of our country, celery is widely concerned about the germination of its seeds, and the use of exogenous plant hormone treatment can break the dormancy and promote seed germination. In this experiment, two celery seeds were treated by different concentrations of Gibberellin (GAs) and Abscisic acid (ABA), the results show that the single factor GAs, ABA, GAs could significantly improve the germination rate and germination potential of celery seed, ABA could significantly inhibit the seed germination. The concentration of 2.00g / L GAs solution treatment, the promotion of celery seed germination strongest, while the ABA concentration is higher, the stronger the inhibition of seed germination. GAs and ABA work together, the concentration is 0.05 g/L, the volume ratio was 1: 1, the enhancement of seed germination is the strongest, and it indicated that the dynamic balance between GAs and ABA was important for celery seed germination.
Key words: Celery；Seed；Gibberellin；Abscisic acid
目 录
摘要 1
关键词 1
Abstract 1
Key words 1
引言 1
1 材料与方法 2
1．1 试验材料 2
1．2 试验方法 2
1．2．1 预处理 2
1．2．2 对照组处理 2
1．2．3 外源GAs、ABA单因素处理 2
1．2．4 外源GAs和ABA协同处理 3
1．2．5 RNA的提取及cDNA的合成 3
1．2．6 芹菜苗中α-淀粉酶基因的克隆 3
1．2．7 序列分析 3
1．2．8 实时定量PCR反应 3
2 结果与分析 3
2．1 数据处理与分析 3
2．1．1 对照组处理 4
2．1．2 外源GAs、ABA单因素处理 4
2．1．3 外源GAs和ABA协同处理 5
2．1．4 芹菜苗中α-淀粉酶基因的克隆 6
2．1．5 实时定量PCR 7
3 讨论 7
致谢 8
参考文献 8
ABA、GAs和温度协同作用对芹菜种子萌发的影响
引言：芹菜(Apium graveolens L.)是伞形科的二年生草本植物，适宜在冷凉环境中生长，最宜的生长温度为10-15℃，原产地为欧洲地中海一带的的沼泽地区。其营养价值非常高，含有丰富的文生素、矿物质以及人体不可或缺的各种蛋白质及膳食纤文，是被公认的重要的天然保健品之一[1]。芹菜作为全球的十大蔬菜之一，在我国的栽培历史也十分悠久，而且分布范围很广，不少地区已经实行排开种植，做到了周年供应[2]。按照《中国农业统计资料》的记载[3]，截止到2008年，全国范围内的的芹菜栽培面积已经达到了860万亩，并有逐年上升的趋势，因此，成为了我国七大重点蔬菜栽培种类之一。