玉米基因组花药特异表达基因的鉴定及功能分类
毕业论文关键词：玉米；全基因组；花粉特异表达基因；功能分类
Genome-wide identification and functional classification of anther-specific genes in maize
Abstract： Breeding and utilization of male sterility lines in maize is an important practical way for hybrid vigor application in maize. The tassel fertility in maize is controlled by a number of genes which regulates cytogenesis and meiosis of microsporocytes, development of anther lobes, tapetum function, pollen wall formation, pollen maturation and anther dehiscence etcetera. The expression disorder or genetic mutation of gene related to tassel fertility may cause anther/pollen functional abnormality, i.e. male sterility. Isolation and function analysis of the key genes controlling anther/pollen development is a basic important research projection for bioengineering for creation of male sterility lines and restoration lines as well as for creation of maintainer lines for nuclear male sterility. This research project searched the gene expression pattern of all the genes in maize genome and found there are 601 anther-specific genes in maize genome, which means just expressed in anther or pollens but not expressed in any other tissues in maize plants. Each chromosome of maize genome contains more than 30 anther-specific genes. For example chromosome 1# has 89, 10# 33. The genes has analyzed by OmicsBean, an enrichment analysis software. All the genes obtained could be related to the biological function of pollens, including viability, tube emergence and double fertilization. Isolation and analysis of anther/pollen-specific genes is an important research to understand the molecular mechanism of male sterility and could be beneficial for hybrid seed production in agriculture.
Key words: maize；genome-wide；anther-specifically expressed genes；functional classification
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法2
1.1花药特异表达基因的获得2
1.2花药特异表达基因染色体分布的统计与分析2
1.3花药特异表达基因的功能分类及玉米雄性不育相关候选基因的筛选3
2结果与分析3
2.1玉米全基因组花药特异表达基因3
2.2玉米花药特异表达基因的功能分类5
2.3分析7
3讨论 7
致谢8
参考文献8
图1 玉米花药特异表达基因 GRMZM2G410990的表达模式图3
图2 玉米花药特异表达基因 GRMZM2G410990的表达量图4
图 3 花药特异表达基因染色体分布图5
图 4 花药特异表达基因GO (GENE ONTOLOGY)功能分类6
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