猪干扰素调控因子5基因的鉴定分析
毕业论文关键词：猪；IRF5；单核苷酸多态性
Identification analysis of porcine interferon regulatory factor 5 (IRF5) gene
Abstract: Interferons (IFNs) are a family of multifunctional cytokines that potently demonstrate antiviral, ant proliferative and immunomodulatory activities. Interferon regulatory factors (IRFs) were identified originally as transcription factors in the regulation of interferon expression and has been received more and more attention for its key role that links innate and adaptive immune response. Interferon regulatory factor 5 (IRF5) gene is one of IRFs family and is also a crucial transcription factor in immune system, it has shown its functionally perse roles in the regulation of antiviral responses. In this experiment, three breeds of pig which include Landrace, Large white and Songliao black pig were selected to analyze the molecular characterization of IRF5 gene firstly. In addition, we designed the specific primer pairs fort exons region of porcine IRF5 gene and identified a single nucleotide polymorphism in exon 7 (g.10764 T>C) by PCR amplification, directly sequencing technology for PCR products and DNAMAN, CHROMAS software. These results should provide scientific reference for the further association study between IRF5 gene and immune traits in pig.
Keywords: Pig; IRF5; SNP;
目录
摘要 2
关键词 2
Abstract 2
Keywords 2
1. 前言 3
1.1 猪IRF5基因的分子结构特点 3
1.2 IRF5基因的研究进展 3
1.2.1 IRF5基因的抑制肿瘤作用 4
1.2.2 IRF5基因在免疫调控中的作用 4
1.2.3 IRF5基因在神经系统中的作用 4
1.3 单核苷酸多态性检测与应用 5
1.3.1 SNP研究 5
1.3.2 SNP标记特点 5
1.3.3 SNP标记的应用 5
1.4 本研究的目的与意义 6
2. 实验材料和方法 6
2.1 实验材料 6
2.1.1 实验动物 6
2.1.2 实验药品和试剂 6
2.1.3 实验仪器 7
2.3 实验方法及步骤 7
2.3.1 引物的合成 7
2.3.2 基因组提取 8
2.3.3 PCR扩增 8
2.3.4 琼脂糖凝胶电泳 9
2.3.5 PCR产物测序 10
3 结果与讨论 10
3.1猪耳组织基因组提取结果 10
3.2 PCR扩增结果 10
3.2.1 不同引物的梯度PCR扩增 10
3.2.2 不同引物的PCR扩增结果 11
3.3 猪IRF5基因的多态性检测 12
4 结论与展望 13
4.1 实验结论 13