三维生物医学组织支架生物海绵制备的关键技术研究
关键词 皮肤组织工程 重组人源胶原蛋白 原花青素 人脐静脉内皮细胞(HUVEC)
毕业论文设计说明书外文摘要
Title Research on the key technique of three dimensional tissue scaffolds fabrication
Abstract
In recent years, People committed to the research of varieties of skin substitutes, for the repair of full-thickness skin defect especially in the treatment of patients suffering from large deep burn. Collagen with good biological characteristics has become a common carrier material for tissue engineering.The recombinant human collagen (RHC) used in this paper is a protein with character of human collagen produced by collagen gene sequence design via gene engineering, followed by expression of Picha Pastoris system. Compared with collagen extracted from animal, it possesses the advantages of hydrophilic without virus risk. In this paper, we used a natural crosslinking agent - proanthocyanidins (PA) cross-linked RHC-chitosan scaffold to investigated its biocompatibility. Through the AO / EB staining and qRT-PCR, we explored the effects of scaffold on proliferation, migration and differentiation of Human Umbilical Vein Endothelial Cells (HUVEC).As a result, the scaffold exhibited non cytotoxicity that it did not affect the cell growth and reflected good cell compatibility. These characterization indicated that this scaffold can be considered as an ideal candidate for skin tissue engineering.
Keywords Skin tissue engineering Recombinant human collagen Proanthocyanidins Human umbilical vein endothelial cells
目 次
1 绪论 1
1.1 胶原蛋白 1
1.1.1 胶原蛋白简介 1
1.1.2 胶原蛋白结构与类型 1
1.1.3 重组人源胶原蛋白简介 2
1.1.4 重组人源胶原蛋白功能与应用 3
1.2 皮肤组织工程 3
1.2.1 组织工程简介 3
1.2.2 组织工程中细胞培养 3
1.2.3 皮肤组织工程发展 4
1.2.4 重组人源胶原蛋白在皮肤组织工程中的应用 5
1.3 胶原三文多孔支架常用交联方法 5
1.3.1 常用交联方法 6
1.3.2 天然交联剂 6
1.4 课题主要研究内容与意义 8
2 重组人源胶原蛋白-壳聚糖生物海绵对HUVEC细胞的影响 9
2.1 材料与方法 9
2.1.1 实验材料与试剂 9
2.1.2 主要仪器及设备 10
2.1.3 细胞二文静态培养 11
2.1.4 细胞三文静态培养 11
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