水稻直穗病与二甲基砷酸积累的关系研究
毕业论文关键词：水稻直穗病；二甲基砷；砷的甲基化；
Study on the Relationship between Straighthead disease and the Accumulation of DMA
Abstract：Straighthead disease, a physiological disorder characterized by sterile florets and distorted spikelets, causes significant yield losses in rice.By previous studies,arsenic is considered as the most important one in straighthead disorder. In the processing of rice for different period of the different arsenic forms, it found that only observed similar Straighthead phenotype when booting stage of rice for DMA treatment. According to the arsenic accumulation and speciation in the disease zone and the text of put the paddy soil in pathogenesis area.We finally found that the accumulation of DMA is the major occurrence of straighthead disease. And the DMA in rice mainly derived from soil, by the process of the microbial arsenic(As) methylation.
Key words: Straighthead; DMA; Arsenic methylation
目录
摘要： 4
关键词： 4
Abstract： 4
Key words: 4
引言： 4
1. 砷的基本性质 5
2. 砷在环境中的生物化学循环 5
2.1. 砷在环境中的迁移途径 5
2.2. 砷在土壤中的生物甲基化与去甲基化 5
2.2.1. 砷的甲基化过程 5
2.2.2. 甲机砷的去甲基过程 5
3. 国内外研究现状 6
实验材料与方法： 6
1. 实验材料： 6
2. 实验方法： 7
2.1 籽粒及颖壳中总砷的测定方法 7
2.2 籽粒及颖壳中砷形态的测定： 7
2.3 土壤孔隙水中砷形态的测定方法： 7
实验一：水稻直穗病田间调查与植物样品分析 8
1. 实验目的 8
2. 采样地点 8
3. 样品分析 8
4. 结果与讨论 8
实验二：直穗病发病区水稻土厌氧培养 11
1. 实验目的 11
2. 供试土壤 11
3. 实验设置 11
4. 结果与讨论 11
实验三： 土壤孔隙水中DMA与水稻积累DMA的相关性（Binding）分析 12
1. 实验目的 12
2. 结果分析与讨论 12
总结 12
讨论 13
参考文献： 14
缩写词英汉对照
As (arsenic) 砷
As(III) (arsenhe) 无机三价砷
As(V) (arsenate) 无机五价砷
MMA(V) (methylarsenate) 单甲基砷
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