外源硫化氢对萝卜芽苗下胚轴中花青苷合成的影响
毕业论文关键词：花青苷；萝卜芽苗；硫化氢；基因表达
The effects of hydrogen sulfide on anthocyanin biosynthesis in hypocotyls of radish sprouts Student majoring in Horticulture
Abstract: In this study, radish sprouts varieties ‘YangHua’ sensitive to anthocyanin were tested to investigate the effects of hydrogen sulfide (H2S) on the synthesis of flavonoids. The influences of H2S on anthocyanin synthesis were studied with different concentrations of exogenous H2S donor, NaHS, treatments and the absence of NaHS was as control. The results showed that the content of anthocyanin was significantly improved as the increasing of NaHS concentration within a certain range(0.5 mM～2 mM). The measures of structural gene and transcription factor expressions showed that the key structure genes,PAL, CHS, CHI, F3H, DFR, ANS, LDOX, UF3GT,and regulator genes, PAP1, PAP2 of anthocyanin synthesis pathway were notably promoted as the increasing tendency of anthocyanin change. Meanwhile, the crucial enzyme activity of PAL and CHI were higher with NaHS treatments than control. All the conclusions showed that H2S developed anthocyanin synthesis and accumulation by regulating the transcription factors and key structural genes stimulated the enzyme activity. Anthocyanin content in radish seedlings was decreased significantly but still higher than the control with the co-treatment of NaHS and PAG, the inhibitor of H2S synthesis,which showed that exogenous H2S by mediating the endogenous H2S content to regulate anthocyanin synthesis.
Key words: anthocyanin; radish sprouts; H2S; gene expression
目 录
摘要 1
关键词 1
Abstract. 1
Key words 1
引言 1
1 材料与方法 3
1.1 试验时间与试验材料 3
1.2 催芽、播种与培养方法 3
1.3 硫氢化钠浓度的选择与确定 4
1.4 花青苷含量测定方法 4
1.5 苯丙氨酸转氨酶（PAL）和查尔酮异构酶（CHI）活力的测定方法 4
1.5.1 PAL酶活检测方法 4
1.5.2 CHI酶活检测方法 4
1.6 CHS酶活检测方法 4
1.7 基因表达量检测方法 5
1.8 抑制剂浓度的选择与确定 5
1.9 数据处理及作图方法 5
2 结果与分析 5
2.1 外源硫氢化钠对萝卜芽苗下胚轴中花青苷含量的影响 5
2.2 外源硫氢化钠处理对花青苷合成途径中关键酶活力的影响 6