三氮唑特效降解菌的表征及降解条件的优化
关键词 三氮唑 特效菌 Shinella sp. 生物降解
毕业论文设计说明书外文摘要
Title Characterization of a new 1,2,4-trizole degrading strain and optimization of the degradation conditions
Abstract
1,2,4-triazole, which is a typical refractory nitrogen heterocyclic compounds, is widely used in medicine, pesticide, chemical and other industries. The identification and characterization of a new triazole degrading strain and its incubation conditions for degradation of triazole were investigated in this paper. The results showed that, the new strain, named NJUST26, which could utilize triazole as the sole carbon, nitrogen and energy source, was identified as Shinella sp.. The strain could completely degrade triazole in pH 5.0-8.0. The optimum temperature and initial pH for triazole degradation were 30℃ and pH 6-7, respectively. The effects of different nutrition condition including various nitrogen and carbon source on triazole biodegradation by NJUST26 were investigated. (I) NH4+ (5mM、10mM、20mM) and NO3- (5mM、10mM) facilitate triazole degradation, while NO2- (5mM、10mM、20mM) and high concentration of NO3- (20mM) provide inhibition effect. (II) Low concentration of yeast (0.5g/L) has the most remarkable effect on triazole degradation, followed by glucose and sucrose. But high concentration of yeast and sucrose will inhibit degradation, through glucose still can accelerate triazole degradation. In addition, the reactor experiment indicated that the dosing of NJUST26 to the reactor system could remarkably enhance the triazole biodegradation ability.
Keywords Triazole Specific bacteria Shinella sp. Biodegradation
目 次
1 引言 1
1.1 三氮唑的简介 1
1.2 三氮唑结构与性质 1
1.3 三氮唑的应用 2
1.4 三氮唑的危害 2
1.5 三氮唑废水的处理方法 2
1.6 课题研究的目标和意义 4
2 材料与方法 5
2.1 实验材料 5
2.2 实验方法 6
2.3 实验表征方法 8
3 结果与讨论 9
3.1 表征与鉴定 9
3.2 影响因素的确定 10
3.3 三氮唑废水生物强化处理 15
结论 17
致谢 18
参考文献 19
附录A Shinella sp. NJUST26的16SrDNA 序列表 22
1 引言
1.1 三氮唑简介
三氮唑是含氮杂环化合物的典型代表，是大量复杂含氮杂环化合物的基本结构单元，是所有细胞和生命物质的一个重要组成部分[1]。近年来，三氮唑因其特有的高生物活性和低毒性，成为医药和农药研究和开发的重要结构组成单元。