复合菌与不同益生菌发酵饲料的对比研究
毕业论文关键词：复合菌；发酵；营养价值
Comparative study of compound probiotics and different probiotic fermented feed
Abstract：Fermented feed has been studying at home and abroad for many years, made some progress. But the study more emphasized on a probiotic fermentation, there are few reports on the fermentation of compound probiotics. In this study, lactic acid bacteria, bacillus and yeast were mixed in proportion as inoculum of corn-soybean meal for fermentation, while three kinds of probiotics were severally used as inoculum for fermentation test. The concentration of lactic acid, nutrients, VFA and the counts of probiotics of fermented feed were compared in this article. The results show that: compared with lactic acid bacteria and bacillus group, the pH value of complex probiotics group was significantly decreased (P<0.05);the concentration of lactic acid of lactic acid bacteria group and complex probiotics group increased, the difference between the two groups was significant (P<0.05). There was no significant difference in the crude protein content between the four groups. The content of crude fat of lactic acid bacteria group was significantly higher than that of the other three groups (P<0.05), and the crude fiber content was not significantly difference between the four groups (P>0.05). Complex probiotics group significantly increased the number of probiotics (P<0.05), the total amount of probiotics was higher than that of the other three groups; the concentration of acetic acid of complex probiotics group was significantly higher than the other three groups (P<0.05); compared with the lactic acid bacteria group and the yeast group, the total VFA of the complex probiotics group was significantly increased (P<0.05). The results revealed that the quality of complex probiotics fermented feed was better than that of single strain fermented feed, which has good development potential.
Keywords：complex probiotics; fermentation; nutritive value
目 录
摘要1
关键词1
Abstract1
Key words2
引言2
1 材料与方法2
1.1 试验仪器及试剂2
1.1.1 试验仪器 2
1.1.2试验试剂和菌种3
1.2 材料的制备3
1.2.1 发酵饲料的制备3
1.2.2 培养基的制备3
1.3 试验方法3
1.3.1饲料pH值和乳酸的测定3
1.3.2饲料常规养分的测定3
1.3.3饲料中微生物数量的测定4
1.3.4饲料VFA的测定4
2 结果与分析4
2.1发酵后饲料pH和乳酸的变化4
2.2发酵后饲料营养成分和干物质化4
2.3发酵后饲料微生物含量变化5
2.4发酵后饲料VFA含量变化5
3 讨论5
4 结论6