细菌硫酸软骨素类发酵工艺条件研究
毕业论文关键字：枯草芽孢杆菌；发酵；优化；提取；测定
Research on fermentation conditions of bacterial chondroitin sulfate
Abstract：Chondroitin sulfate is a kind of acid mucopolysaccharide which has many important biological activity and is widely used in medicine, cosmetic and food, etc. It is of great significance in theory and practice to improve the market competing capacity and expand application area through the research of chondroitin sulfate with high production,inexpensive price,short-cycle and good performance.The main contents of this paper were as following:the fermentation technology of bacterial chondroitin sulfate polysaccharide,the optimization fermentation condition of bacterial chondroitin sulfate polysaccharide , the extraction technology of bacterial chondroitin sulfate , the establishment of the assaying methods of bacterial chondroitin sulfate,which would lay a foundation for further study on chondroitin sulfate.In the fist part of this thesis,by using of single factor experiment and orthogonal experiment,we determined the optimal ratio of the culture medium,that is,sucrose 2.0%，nitrogen sources(peptone-yeast extract) 2.%,，NaCl0.1%,，KH2PO4 0.2%，MgSO4 0.15%。Meanwhile,single factor experiment was applied to optimize shaking culture,including temperature-28℃,rotational speed of shaking tables-160rpm,inocul-m dose-4%and fluid volume-50mL.Based on the optimal medium,we obtained Chondroitin Sulfate with the yield of 0.88g/L.A subsequent 5L-scale fermentation improved the yield to 1.2g/L.
Key words：bacillus subtilis；fermentation；optimization；extraction；assaying
目录
1. 绪论 1
1.1 硫酸软骨素概述 1
1.1.1 硫酸软骨素结构组成 1
1.1.2 硫酸软骨素物理性质 1
1.1.4 硫酸软骨素作用与用途 2
1.2 枯草芽孢杆菌 2
1.3 微生物发酵法 3
1.4 提取方法研究进展 3
1.5 分离纯化 3
1.6 结构分析方法 5
1.7 硫酸软骨素研究的内容及意义 5
2. 材料与方法 6
2.1 菌种 6
2.2 主要试剂与药品 6
2.3主要实验仪器 7
2.4 实验培养基及主要试剂的配制 7
2.5 方法 8
2.5.1 菌种的活化 8
2.5.2 生理生化鉴定 8
上一篇：籼稻蛙卵胚诱导发生体系研究