土壤重金属Cd、Al单一及其复合胁迫对矮牵牛幼苗生长的影响及其机理
毕业论文关键词：Cb；Al；胁迫；矮牵牛幼苗；
Soil Heavy Metals Cd, Al and Their Compound Impact of Stress on Seedling Growth of Petunia and Mechanism
Abstract: In this study, two weeks growing petunia seedlings are regarded as the basic material of the experiment, using of different concentrations of Cd, Al and Combined Stress treatments to analyses the changes of growing petunia seedlings. Throughout the course of the experiment, we mainly focus on the following aspects of the recording and analysis, including chlorophyll, soluble protein, soluble sugar, POD, MDA, SOD content, superoxide anion production rate and root activity. The results show that under the stress of single Cd, the soluble sugar, POD, MDA, SOD content and superoxide anion production rate are rising with the increasing of Cd concentration. On the contrary, with the increasing of concentration, the chlorophyll, soluble protein content and root activity will be decreasing.
Cd has more influences on the physiological and biochemical Petunia than Al. Petunia has poor ability of resistance for Cd contamination. The protective effects of SOD at high concentrations is more obvious under heavy metals stress, but the protective effect of CAT and POD is relatively weak. Combined stress of petunias have more influences for Cd and Al than the single stress. Combined and single stress of petunias is also mutually reinforcing.
Keywords: Cb; Al; Stress; Petunia Seedlings
目 录
摘 要： 1
引言 2
1材料与方法 3
1.1材料及处理方法 3
1.2实验设计 3
1.3实验测定方法 4
1.4实验数据分析 4
2结果与分析 4
2.1Cb、Al单一及其复合胁迫对矮牵牛生物指标的影响 4
2.2Cb、Al单一及其复合胁迫对矮牵牛可溶性糖含量的影响 6
2.3Cb、Al单一及其复合胁迫对矮牵牛叶绿素、可溶性蛋白含量的影响 7
2.4Cd、Al单一及其复合胁迫对矮牵牛MDA和SOD、POD、CAT活性的影响 8
2.5Cb、Al单一及其复合胁迫对矮牵牛的超氧阴离子产生速率影响 11
2.6Cb、Al单一及其复合胁迫对矮牵牛根系活力的影响 11
3讨论 12
参考文献 13
致谢 16
土壤重金属Cd、Al单一及其复合胁迫对矮牵牛幼苗生长的影响及其机理 引言
矮牵牛(Petuniahybrida)又称碧冬茄，毽子花，也有人们亲切的称之为矮喇叭。矮牵牛属于被子植物门茄科碧冬茄属植物。矮牵牛花朵硕大，色彩丰富，花型变化颇多，花期长，而且还能做到周年繁殖上市，是重要的观赏花卉之一，在欧美及日本等地区广泛栽培[1]。除了可用于制造花坛、花境外，也可作盆栽花卉、吊篮或花钵栽培，近年来在城市园林绿化中扮演着越来越重要的角色。矮牵牛为多年生草本，茎直立或匍匐，花朵呈漏斗状，花瓣边缘变化大，花色有白、粉、红、紫、蓝、黄等。矮牵牛是以在舒适的温度下生长，而这个舒适的温度介于13摄氏度与18摄氏度之间。夏季可以承受的温度高达35摄氏度。夏季属于矮牵牛的生长发育旺盛季节，一定要注意保证其能够吸收到充足的水分，可以的办法有定期定时定量的浇水，保持其有个潮湿并且利于生长的环境。
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