安徽九华山植物内生真菌的分离及其抗菌活性的初步研究
毕业论文关键词：九华山 1，内生真菌 2，抗菌活性 3， 筛选 4
Isolation of Endophytic Fungi from jiuhua mountain and Its Antimicrobial Activity
Abstract：Objective To isolate endophytic fungi of plant of jiuhua mountain, these endophytic fungi were screened with antibacterial activity. Method cut the plant samples into small pieces,then inoculate these samples into potato dextrose agar solid medium, wait until they grow into mycelium, so we can isolate，purified and store them.These purified culture was fermentated and these culture broth was filtered.So that we can get zymotic fluid and mycelium,which then were respectively dissolved into the 70% ethanol.these solution was filtered again and dissolved with DMSO to give endophytic fungal metabolite extracts, To take 96 test method on these extracts to find whether these extrats have inhibitory or not with Staphylococcus aureus, Escherichia coli and Bacillus subtilis. Results the number of screened fungi which have significant inhibitory effect (which means these inhibition rate more than 50%) is 16,and these strains were: A2, A6, A9, B1, B6, B7, C5, D8, D10, D12, E1, E2 , E3, E7, G2, H3. Conclution The plant of jiuhua mountain have variety,abundant endophytic fungi resources,and these fungi has a significant inhibitory effect.So it has enormous potential for the study of new type of medical drug
Key words jiuhua mountain 1;endophytic fungi 2;Antimicrobial Activity 3;screen 4
目录
引言4
1 材料 5
1.1 植物材料 5
1.2 供试指示菌 5
1.3供试培养基 5
1.4实验试剂 5
1.5实验器材 5
2 方法 6
2.1 内生真菌的分离 6
2.2 去除重复菌株 6
2.3内生真菌的发酵 6
2.4 内生真菌代谢产物的提取及抑菌活性检测 7
3 结果 7
3.1 内生真菌的分离 7
3.2 内生真菌代谢产物的抑菌活性 8
4 讨论 9
致谢 10
参考文献 10
引言
内生菌的概念最早是由DE Bary(1866)首次提出，包括内生细菌、内生放线菌和内生真菌等[1-2]。其中内生真菌是指在其生活史中一段时期生长在植物组织内而对其没有明显病害的一类真菌[3]。根据内共生理论，在共生体内一旦出现某种有用的代谢产物，它就能够被其他生物所用，并且表现为相互作用和“协同共生”。最早分离得到的内生真菌是由Vogl在1898年从黑麦草的种子种分离得来的，自此可知关于内生真菌的研究已经有将近100多年的历史了[4]，然而从1890年至1980年之间，除了少数有关内生真菌的报道意外，很少有人从事这一领域的研究，一直到80年代初期内生真菌才逐渐被人重视起来。目前，随着内生真菌研究的不断深入，为新型医用药物的研制提供了新的开发材料。
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