花马池盐湖嗜盐细菌多样性研究
毕业论文关键词：454高通量测序；嗜盐细菌；多样性；盐湖；16S rRNA
The Research of Halophilic Bacteria Diversity in HuaMaChi Salt Lake
Abstract: The experimental study of halophilic bacteria using PCR amplification of total gene sequences , using 454 high-throughput sequencing methods to detect the 16S rRNA gene sequences. After searching in NCBI database, using taxonomic methods to analyse halobacteria persity of Huamachi salt lake. The final statistical analysis for the 26698 optimization sequence and 207 OTU . The sequence of halophilic bacteria can be classified as 10 phyla, 15 classes, 31orders, 56 genera. The environmental advantages of microflora are the Firmicutes, Proteobacteria, Bacilli, Gammaproteobacteria, Lactobacillales, Pseudomonadales , Lactococcus ,respectively, of the total sequence of 24.12%, 20.01%, 23.06%, 19.42%, 20.75%, 7.58%, 18.81% . On the level of genus, Up to 44.45% Sequence of samples have yet to be named.
Keywords: 454 sequencing; halophilic bacteria ; persity ; salt lake; 16S rRNA
目录
引言 1
1 材料与方法 2
1.1实验材料 2
1.1.1样地概况 2
1.1.2获取水样 2
1.2具体实验流程 2
1.2.1宏基因组DNA提取 2
1.2.2 宏基因电泳检测与分析 2
1.2.3 设计并合成引物接头 2
1.2.4 16S rRNA基因的PCR扩增和产物纯化 3
1.2.5 PCR扩增产物定量和均一化—荧光定量 3
1.2.6 454 pyrosequencing测序 3
1.3测序数据处理与统计 3
1.3.1 有效序列数据处理与统计 3
1.3.2优化序列数据处理与统计 3
1.4 分类单元OTU统计与分析 3
1.5 群落组成分析 4
2实验结果与数据分析 4
2.1 454测序结果 4
2.2 嗜盐细菌的丰度对比 4
2.3嗜盐细菌的多样性指数分析 5
2.4嗜盐细菌多样性特征分析 6
2.4.1嗜盐细菌在门水平上的多样性分析 6
2.4.2嗜盐细菌在纲水平上的多样性分析 7
2.4.3嗜盐细菌在目水平上的多样性分析 8
2.4.4嗜盐细菌在属水平上的多样性分析 10
3.讨论 12
致谢 13
参考文献 13
引言