徐州鱼塘细菌多样性研究
毕业论文关键词：多样性；阳性菌；阴性菌；致病菌；16S rRNA；系统发育树
Study on bacterial persity in fish pond water in the north of Xuzhou
Abstract：Healthy breeding of fish ponds restricted by many factors, such as the types of fish and fish ponds area size, etc. At the same time, the bacteria in fish pond water for fish survival but also bring significant impact .This paper studies the influences of different bacteria to different fishes in the fish pond in the north of Xuzhou. The research is mainly through 16S rRNA gene sequencing of the fish pond water bacteria in the north of Xuzhou, and the 16S rRNA gene sequence analysis of phylogenetic tree construction, finally get the composition of bacterial species in water. After testing , We found that there were nine kinds of bacteria in fish pond water, they were Bacillus horikoshii、Aeromonas punctata、Bacillus thaonhiensis、Staphylococcus haemolyticus、Shigella flexneri、Bacillus indicus、Fictibacillus phosphorivorans、Enterobbacter soli、Bacillus aryabhattai. The Aeromonas punctata、Staphylococcus haemolyticus and Shigella flexneri were pathogenic bacteria.Study on the effect of bacterial persity and pathogen on fish survival status of the fish pond water in the north of Xuzhou can not only improve our microbial species resources of aquiculture environment, but also provide an important reference for the healthy breeding of the fish ponds.
Key words: persity; Gram-negative bacteria; Gram-positive bacteria; pathogenic bacteria; 16S rRNA; Phylogenetic tree
目录
引言 1
1材料与方法 2
1.1 材料 2
1.1.1 水样采集 2
1.1.2 配制培养基 2
1.1.3 主要仪器与试剂 2
1.2 方法 2
1.2.1 对菌种进行划线分离与纯化并斜面保存 2
1.2.2 Gram stain染色 2
1.2.3 细菌总DNA抽提及电泳分析 2
1.2.4 细菌的PCR扩增 2
1.2.5 PCR扩增产物电泳分析 3
1.2.6 扩增产物胶回收 3
1.2.7 测序 3
1.2.8 构建系统发育树 3
2结果与分析 3
2.1 革兰氏染色结果 3
2.2 系统发育树 3
3讨论 5