外源AsA对涝渍胁迫下芝麻幼苗抗氧化防御和有机渗透调节物质的影响
毕业论文关键词：涝渍胁迫；AsA；芝麻幼苗；抗氧化防御；有机渗透调节
Exogenous Ascorbic Acid on Sesame Seedling under Waterlogging Stress and Antioxidant Defenses of Organic Osmolytes
Summary:The experimental study of different concentrations of exogenous (0,0.1,0.2,0.3,0.4,0.5g / L) of ascorbic acid (AsA) waterlogging stress on the growth of seedlings sesame antioxidant defense capacity and organic osmolytes in capacity. The results showed that: exogenous AsA sesame seedlings can increase soluble protein, free proline and soluble sugar content, improve the ability of osmotic adjustment sesame seedlings; reduce the MDA content accumulated in sesame seedlings, improved anti-sesame seedlings oxidase system activity, reducing the activity of oxygen on sesame seedlings hazards, reducing the membrane lipid peroxidation, maintaining the stability of the membrane system to ease the sesame seedling waterlogging caused by injury, increased chlorophyll content, and enhance the root activity; improve the POD activity, maintain the stability of sesame body of the cell membrane, weakening the membrane lipid peroxidation of sesame seedling injury. Wherein when the concentration of exogenous AsA 0.8g / L best.
Key words:Waterlogging;Ascorbic acid;Sesame seedling;Antioxidant defense; Organic osmotic adjustment
目 录
摘 要 1
引言 2
1材料和方法 2
1.1材料培养及处理 2
1.2测定项目和方法 3
1.3数据分析 3
2结果与分析 3
2.1外源AsA对渍涝胁迫下芝麻幼苗叶片中可溶性蛋白含量的影响 3
2.2外源AsA对渍涝胁迫下芝麻幼苗叶片中游离脯氨酸含量的影响 4
2.3外源AsA对渍涝胁迫下芝麻幼苗叶片中可溶性糖含量的影响 4
2.4外源AsA对渍涝胁迫下芝麻幼苗叶片内MDA含量的影响 5
2.5外源AsA对渍涝胁迫下芝麻幼苗叶片内叶绿素含量的影响 6
2.6外源AsA对渍涝胁迫下芝麻幼苗根系活力的影响 7
2.7外源AsA对渍涝胁迫下芝麻幼苗叶片中SOD活性的影响 7
2.8外源AsA对渍涝胁迫下芝麻幼苗叶片中POD活性的影响 8
2.9外源AsA对渍涝胁迫下芝麻幼苗叶片中CAT活性的影响 9
3讨论 10
4结论 11
参考文献 11
致谢 14
外源AsA对涝渍胁迫下芝麻幼苗抗氧化防御和有机渗透调节物质的影响 引言
环境胁迫严重的危害了植物作物的生长和发育，导致植物作物的产量大幅度下降，植物不能够躲避这些不利的环境因素影响，因此需要它们快速的感应这些胁迫刺激，从而适应这些环境的胁迫[1]。植物对各种环境胁迫的耐受能力和适应性机理一直是人们研究的热点。在众多影响植物生长发育的环境因素中，水分条件是一个重要因素。植物在一定的水分条件下，才能正常进行生命活动[2]。渍涝胁迫的本质之一就是由于缺氧而引起的生理性失调[3]。而芝麻幼苗属于浅根系作物，对于渍涝胁迫更加敏感。
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