紫苏醛对黄曲霉的细胞周期与细胞凋亡的影响
毕业论文关键词：紫苏醛；黄曲霉；细胞周期；细胞凋亡
The Influence of Perillaldehyde on Cell Cycle and Cell Apoptosis of Aspergillus Flavus
Abstract: A. flavus is one of the main reasons for the deterioration of food, control the food corruption caused by food safety problem is very necessary. Perillaldehyde, which is one of the essential oil extracted from perilla by perillaldehyde influence on aflatoxin cell cycle and cell apoptosis, and to explore the effect of perillaldehyde resistance to A. flavus mechanism, plays a key role for the further development of natural antiseptic. This experiment mainly through the ergosterol content determination of perillaldehyde to A. flavus cell membrane has the function to determine whether the perillaldehyde to A. flavus, thus further study on different concentrations of perillaldehyde on aflatoxin cell cycle and cell apoptosis. Results indicated that ergosterol experiment, the resulting data show the ergosterol content and decreased with the increase of concentration of perillaldehyde. In perillaldehyde to A. flavus cell cycle experiment, with the increase of the time, it can be seen that the cell cycle is more and more focused on G1 phase, this shows that perillaldehyde can affect the A. flavus cell pision; it also can be seen that in the apoptosis of experiments perillaldehyde can cause early apoptosis of A. flavus. This series of experiments for the development of new natural preservatives have important theoretical guiding significance.
Keywords: Perillaldehyde; A. flavus; Cell cycle; Cell apoptosis
目录
引言 1
1 材料与方法 3
1.1 供试材料 3
1.1.1 原料 3
1.1.2 主要试剂 3
1.1.3 主要仪器 3
1.1.4 实验试剂的配制 4
2.1实验步骤 5
2.1.1 黄曲霉菌种的活化与接种 5
2.1.1 孢子悬浮液的制备 5
2.1.3 紫苏醛对黄曲霉麦角甾醇的影响 5
2.1.4 紫苏醛对黄曲霉细胞周期的影响 6
2.1.5 紫苏醛对黄曲霉细胞凋亡的影响 6
2 实验结果与分析 8
2.1 麦角甾醇实验的结果与分析 8
2.2 细胞周期结果与分析 9
2.3 细胞凋亡结果与分析 11
3 讨论 12
4 致谢 13
参考文献 14
紫苏[Perilla frutescens (Linn.) Britt]是一种具有重要的生理功能和保健价值的植物，既可药用也可当蔬菜食用，在我国有悠久的历史，属唇形科一年生草本植物，又名苏子、回回苏、红紫苏、红苏、赤苏等，世界各地均有种植[1]。紫苏籽油中含有人体所需八种氨基酸，同时还含有婴幼儿所需九种必须氨基酸，含有蛋白质、β-胡萝卜素、VB1、VB2、VC、PP、Ca、Zn、Fe、Cu、Mo、Mn等多种活性元素及矿物质[2,3,4]。紫苏中含有多种黄酮类、甙类、萜类等化合物，具有调节血脂、降血压、抗氧化和抗癌等作用。Gisho研究发现，紫苏精油中的紫苏醛有抑制细菌生长的作用[5]；Kang等通过对紫苏精油抗菌活性进行研究，发现其中紫苏醛对多种细胞有抑制作用，能有效防止由沙门氏菌属引起的海产品食物中毒[6]；Koga等认为紫苏醛与其他丁香油酚可以有效的抑制绿脓杆菌的传染[7]。