红茶菌混合菌种的分离与鉴定
毕业论文关键词： 红茶菌；混合菌种；分离；鉴定
Vendors of mixed bacteria isolation and identification
Abstract: Vendors have the special nutritional value, drinking vendors can cure some diseases have appeared at home and abroad, the instance of the even is regarded as a kind of multifunctional drug. It is a sugar tea as raw material, the acetic acid bacteria, yeast and lactic acid bacteria and other microorganism fermented together. In the study of vendors, the purification and identification of strains of vendors is the basis of the research vendors, for vendors to produce play an important role. Studies show that by vendors strains of yeast, acetic acid bacteria and lactic acid bacteria, the three kinds of bacteria. This article through to the cultivation of the vendors mixed bacteria and yeast from black tea sugar water, acetic acid bacteria and lactic acid bacteria isolation and identification, etc to study of vendors. Through experiment, cultivate the vendors mixture, and by absorbing a small amount of bacterium fluid from mixture to the separation of different cultures, under the optimum temperature for their cultivation, the yeast strains were Y1, Y2,Y3. Acetic acid bacteria strains A1, A3; Lactic acid bacteria strains of L2, L4. And the related characteristics of strains for research.
KeyWords： Vendors；mixed culture；isolation；identification
目录
1 绪论 1
1.1 本课题的国内外研究进展 1
1.2 本课题的研究内容、目的及意义 3
2 材料与方法 7
2.1 实验材料 7
2.1.1 菌种 7
2.1.2 实验试剂 7
2.1.3 主要仪器和设备 7
2.1.4 培养基及试剂的制备 8
2.2 实验方法 9
2.2.1 红茶菌混合菌种的培养 9
2.2.2 红茶菌混合菌种中酵母菌的分离与鉴别 10
2.2.3 红茶菌混合菌种中醋酸菌的分离与鉴别 10
2.2.4 红茶菌混合菌种中乳酸菌的分离与鉴别 12
3 结果与讨论 13
3.1 红茶菌混合菌种的培养 13
3.2 红茶菌混合菌种中酵母菌的分离与鉴别 13
3.2.1 红茶菌混合菌种中酵母菌的分离 13
3.2.2 红茶菌混合菌种中酵母菌的鉴别 14
3.3 红茶菌混合菌种中醋酸菌的分离与鉴别 16
3.3.1 红茶菌混合菌种中醋酸菌的分离 16
3.3.2 红茶菌混合菌种中醋酸菌的鉴别 16
3.4 红茶菌混合菌种中乳酸菌的分离与鉴别 19
3.4.1 红茶菌混合菌种中乳酸菌的分离 19
3.4.2 红茶菌混合菌种中乳酸菌的鉴别 19
4 结论 23
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