利用运动发酵单胞菌发酵水稻秸秆及甘薯生产燃料乙醇的响应面法优化
毕业论文关键词：运动发酵单胞菌，水稻秸秆，甘薯，乙醇，响应面分析
Optimization of Fuel Ethanol Fermentation Using Rice Straw and Sweet Potato by Zymomonas mobilis through Response Surface Method
Abstract: Fuel ethanol fermentation using rice straw and sweet potato by Zymomonas mobilis was optimized through response surface methodology. First by Plackett Burman design selected factors had significant effect on ethanol content: temperature, pH, ammonium sulfate, soybean powder, glucose, inoculation amount, ratio of sweet potato to rice straw, fermentation time; Approaching maximum ethanol content area through the steepest ascent experiment: The temperature is 38 ℃, pH is 7, ammonium sulfate is 1.5%, 7% content of soybean meal, glucose content is 3%, the inoculation amount is 3.2 OD/g, ratio of sweet potato to rice straw of 5:1, number of days for seven days; Finally, through the Box - behnken design and response surface analysis method is used to determine Zymomonas mobilis fermentation of rice straw to produce fuel ethanol and sweet potato best conditions: 38 ℃ fermentation temperature, inoculation amount OD / 3.2 g, 2.5% ammonium sulfate, pH7, 7% soybean meal, glucose 3%, sweet potato to rice straw is 5, the number of days 7 days, predict the ethanol concentration is 15.8349. Response surface experiment was carried out using the Box - Behnken, by experimental data fitting the response surface model, get the quadratic polynomial, Its binary regression equation model is: Y=9.9812-0.优尔6x1-0. 1718x2-0.06154x3+0. 5762x12-0.3465x22-0. 0486x32+0. 2726x1x2--0.5265x1x3-0.3074x2x3.
Key words: Zymomonas mobilis，Rice straw，Sweet Potato，ethanol，Response Surface Analysis ( RSA )
目录
引言 1
1.材料和方法 3
1.1材料 3
1.1.1试剂及配制 3
1.1.2菌株 3
1.1.3培养基 3
1.1.4仪器设备 4
1.2试验方法 4
1.2.1菌种复苏方法 4
1.2.2培养基的配制 4
1.2.3接种 5
1.2.4乙醇含量的测定 5
1.3实验设计 5
1.3.1 Plackett-Burman设计 5
1.3.2 最陡爬坡实验 5
1.3.3 Box-Behnken设计 6
2.结果与分析 6
2.1 Plackett-Burman设计的结果 6
2.2最陡爬坡实验结果 8
2.3 Box-Behnken设计结果 8
3.结论 11
4.讨论 11
参考文献 12
引言
本实验的研究目的是运动发酵单胞菌采用发酵法利用水稻秸秆和甘薯产生数量可观的燃料乙醇[1] [2]。乙醇渗透了人们生活的各个方面，广泛地应用于食品饮料、香精香料、医药领域。它是一种优良的化工基本原料,同时，它又是一种难得的清洁、方便、安全的能源[3]。燃料乙醇是自然界难得的一种可再生的无限闭路循环的能源，既可以直接最为燃料使用，又可以兑汽油作为混合燃料使用，这样就可以减少不可再生能源的消耗，降低燃烧中的污染物的排放[4] [5]。