碱胁迫对草莓渗透调节物质和保护酶活性的影响
毕业论文关键词：碱胁迫；草莓；渗透调节物质；保护酶
Effects of Alkali Stress on Osmotic Adjustment Solute and Protective Enzyme of Strawberry
Abstract: In order to study the effects of alkali stress on osmotic adjustment solute and protective enzyme of strawberry, strawberries were stressed by different degrees of alkali stress. It also can study the alkali-tolerant physiological mechanism of strawberry and provide theoretical basis for artificial cultivation of strawberry. While strawberries grown to 5-6 leaves, they were treated by NaHCO3 stress with degrees of 0.1%, 0.2% and 0.3%. Then select the 3th-5th function leaves to determine related
physiological indexes after treated for 0, 2, 4, 6 days. The results showed that with the stress degree and the stress time increasing, the physiological indexes of strawberry seedlings changed obviously. The contents of soluble sugar, proline and MDA increased significantly; the activities of protective enzyme (SOD, POD) increased at first and then decrease. These indicated that alkaline stress affected the physiological indexes of strawberry, and increasing osmotic adjustment solute and activities of protective enzymes could enhance the stress-resistance.
Keywords: alkali stress; strawberry; osmotic adjustment solute; protective enzyme
碱胁迫对草莓渗透调剂物质和保护酶活性的影响
目 录
引言 1
1 材料与方法 2
1.1供试材料 2
1.2 主要试剂 2
1.3 仪器设备 2
1.4 测定方法 3
1.4.1 可溶性糖含量的测定 3
1.4.2 游离脯氨酸含量的测定 3
1.4.3 MDA含量的测定 4
1.4.4 SOD活性的测定 4
1.4.5 POD活性的测定 4
2 结果与分析 5
2.1 NaHCO3碱胁迫对草莓幼苗渗透调节物质的影响 5
2.1.1 NaHCO3碱胁迫对草莓幼苗可溶性糖含量的影响 5
2.1.2 NaHCO3碱胁迫对草莓幼苗脯氨酸含量的影响 5
2.2 NaHCO3碱胁迫对草莓幼苗膜脂过氧化作用和保护酶的影响 6
2.2.1 NaHCO3碱胁迫对草莓幼苗MDA含量的影响 6
2.2.2 NaHCO3碱胁迫对草莓幼苗SOD活性的影响 7
2.2.3 NaHCO3碱胁迫对草莓幼苗POD活性的影响 7
3 讨论 8
致谢 9
参考文献 9
引言
随着科学技术不断进步，工业化建设不断发展，环境也随之恶化，土壤盐碱化日益加重，限制农作物的生长发育，降低土地利用率，影响着农业生产和生态环境。盐碱胁迫尤其是碱胁迫，会影响植物的新陈代谢，严重的盐碱土壤地区植物几乎不能生存。据不完全统计，全球盐碱土总面积约为10亿hm2，而中国盐碱土面积约为1亿hm2。在这大范围的盐碱地中，很多情况同时具有盐土和碱土，即复合型盐碱土[1]。颜宏指出，碱胁迫与盐胁迫具有不同性质，既有相同点也有不同点[2]。所以碱胁迫是农业生产和生态环境的重要问题之一，影响着植物的生长发育，而目前这方面的报道相对较少[3-4]。
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