产精氨酸脱亚胺酶工程菌的固定化
关键字：工程菌 固定化 产精氨酸脱亚胺酶 精氨酸 瓜氨酸
毕业设计说明书（毕业论文）外文摘要
Title Production engineering arginine deiminase immobilized bacteria
Abstract
This paper explores the catalytic arginine deiminase pure strain culture method citrulline engineering bacteria and immobilization methods produce while using silica gel thin layer chromatography method for detecting the generation of citrulline and arginine consumption. Engineered bacteria after screening and induction training with arginine deiminase activity, polyvinyl alcohol, sodium alginate and immobilized engineering bacteria to achieve a catalytic arginine deiminase generate citrulline. The experiment project arginine producing bacteria produce deiminase is an intracellular enzyme, the formation of large inclusion bodies after expression of engineering bacteria, making the extraction of cells producing arginine deiminase Arginine deiminase catalytic less efficient, so efficient catalytic method immobilized by arginine deiminase engineered bacteria immobilized and recovery methods to improve engineering bacteria engineered bacteria to reach a better utilization utilization prospects. Meanwhile, arginine and citrulline price disparity, the catalytic generation of citrulline in the field of medical, pharmaceutical precursors, such as the majority of the market, so the production of arginine deiminase immobilized engineered bacteria have developed a greater application prospects and economic value.
Keywords: engineering bacteria, immobilized, arginine deiminase produced citrulline, Arginine, citrulline
目 次
1 引言 1
1.1 精氨酸介绍 1
1.2 瓜氨酸概况 1
1.3 本次研究的意义 3
2 精氨酸脱亚胺酶 4
2.1 精氨酸脱亚胺酶活性的检测 4
2.2 产精氨酸脱亚胺酶工程菌活性的检测 5
3 实验材料和试剂 6
3.1 主要仪器设备 6
3.2 主要原料试剂 7
3.3 实验菌株 8
3.4 常用溶液 8
4 实验方法和结果表征 8
4.1 筛选培养基的制备 8
4.2 工程菌的活化 9
4.3 工程菌的种子培养 9
4.4 发酵培养和诱导 10
4.5 工程菌产精氨酸脱亚胺酶活性的检测 10
4.6 工程菌发酵生产瓜氨酸的探究 10
5 产精氨酸亚胺酶工程菌的固定化 14
5.1 菌株 14
5.2 主要材料和试剂 15
5.3 工程菌固定化方法 15
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