2.5 14个牛品种的聚类分析 18

3 讨论 19

3.1微卫星位点选取问题 19

3.2遗传距离和亲缘关系 19

3.3前景展望 20

4 结论 21

参考文献 21

 致谢 22

图清单

图序号 图名称 页码

图1-1 主要仪器设备 8

 Figure 1-1 Apparatus in present study 8

图1-2 微卫星PCR扩增体系 12

Figure 1-2 Microsatellite PCR amplification system 12

图1-3 微卫星PCR扩增程序 12

Figure 1-3 The Procotol of microsatellite PCR Reaction 12

图2-1 1.5%的琼脂糖电泳检测提取的DNA 14

Figure 2-1 1.5% agarose electrophoresis of genomic DNA 14

图2-2 微卫星位点CSSM66PCR扩增产物琼脂糖凝胶电泳检测 14

Figure 2-2 PCR amplification product detection of Microsatellite loci CSSM66 by agarose gel electrophoresis 14

图2-3 HEL1位点电泳扩增图 14

Figure 2-3 Electrophoresis patterns of representive PCR products of HEL1 Loci 14

图2-4 CSSM66位点电泳扩增图 15

Figure 2-4 Electrophoresis patterns of representive PCR products of CSSM66 Loci 15

图2-5 ETH10位点电泳扩增图 15

Figure 2-5 Electrophoresis patterns of representive PCR products of ETH10 Loci 15

图2-6 ILSTS06位点电泳扩增图 15

Figure 2-6 Electrophoresis patterns of representive PCR products of ILSTS06 Loci 15

图2-7 MM8位点电泳扩增图 15

Figure 2-7 Electrophoresis patterns of representive PCR products of MM8 Loci 15

图2-8 MM12位点电泳扩增图 15

Figure 2-8 Electrophoresis patterns of representive PCR products of MM12 Loci 15

图2-9 INRA005位点电泳扩增图 16

Figure 2-9 Electrophoresis patterns of representive PCR products of INRA005 Loci