摘要：从虎杖中提取白藜芦醇的粗提取液，应用响应面法（RSM）对双水相萃取法分离提纯工艺进行优化。以单因素实验作为基础，选取丙酮浓度、硫酸铵浓度、pH为三个主要影响因素，进行Box-Behnken 中心组合试验设计，优化虎杖中白藜芦醇的提取工艺条件。结果表明，响应面法对白藜芦醇的粗提取液的分离提纯萃取的最优条件为：丙酮浓度62.8%、硫酸铵浓度22.10%、pH5.92，白藜芦醇的萃取率预测值95.62%。实验得到白藜芦醇的萃取率94.98%，与预测值的相对误差为0.67%。说明该优化方案具有一定的可行性。49818

毕业论文关键词：虎杖；白藜芦醇；双水相萃取；响应面法

Study on the Extraction Technology of Resveratrol

Abstract: Extract resveratrol of crude extract from giant knotweed, then using the Aqueous two-phase system, separation and purification of resveratrol, applying the response surface method (RSM) optimized the extraction process of resveratrol. By single factor experiment as the foundation, choosing acetone concentration, concentration of ammonium sulfate, pH for the three main factors of Box - Behnken center combination experiment design, optimization of the extraction process of resveratrol in the giant knotweed conditions. The results showed that the method of responsed surface method for separation and purification of the crude extract resveratrol extraction of the optimal conditions for: acetone concentration 62.8%, 22.10% ammonium sulfate concentration, pH5.92, resveratrol extraction rate predictived value of 95.62%. Resveratrol was obtained by experiment after three times the average extraction rate was 94.98%. And predictive value of relative error was 0.67%, showed that the optimized scheme has certain feasibility.

Keywords: Polygonum cuspidatum; Resveratrol; Aqueous two-phase Extraction; Response Surface Method

目  录

摘要 1

关键词 1

引言 2

1 材料与方法 2

1.1 实验材料与仪器 2

1.2实验方法 3

1.2.1配制标准品溶液 3

1.2.2检测最适波长 3

1.2.3白藜芦醇标准曲线 3

1.2.4原材料处理 3

2 实验结果分析 4

2.1单因素分析 4

2.1.1不同丙酮浓度对白藜芦醇萃取率的影响 4

2.1.2不同硫酸铵浓度对白藜芦醇萃取率的影响 4

2.1.3不同pH对白藜芦醇萃取率的影响 5

2.1.4不同温度对白藜芦醇萃取率的影响 5

2.2关键因素的选取 6

2.3 响应面法对双水相法萃取白藜芦醇工艺参数的优化 6

2.3.1 设计方案及相应值结果 6

2.3.2 白藜芦醇得率回归模型的建立和显著性检验 7

2.3.3白藜芦醇萃取率的响应面分析 8

2.3.4白藜芦醇最佳提取条件的确定及可靠性验证 9

3 结论与讨论 9

参考文献 11

致谢 13 

白藜芦醇分离提取工艺研究