木质素降解菌CY的分离鉴定及其生长特性研究
参考文献 20篇。 毕业论文关键词：木质素 海粘假交替单胞菌 分离 鉴定 生长特性
Isolation, identification and growth characteristics of a lignin degrading strain CY Abstract This thesis reported the isolation and characterization of a bacterial stain named CY that has the ability to use lignin as sole carbon source for growth from the Lianyungang fresh seawater. Up to 42.31 % of 50 mg/L lignin was degraded by this bacterial under the condition of 30°C, 250 r/min within. By the colony of Gram staining observation, electron microscopy, and its morphological observation by serological test, physiological and biochemical identification. On the basis of the results of the PCR amplification and 16SrDNA sequencing of its genome sequence, and EzBioCloud site included with high homology strains were phylogenetic analysis and by MAGE 5.1 method (Neighborhood-joining) to construct adjacency relation analysis and phylogenetic tree. In addition, the growth characteristics was studied. Experimental results showed that, the strain CY cells are gramstaining- negative, non-flagellated. They are globular, 0.84 µm in diameter. After 2 days of incubation on seawater solid nutrient agar, colonies grew to milky yellow, smooth edge, uplift, round, thick, easy picking. strain CY 16S rDNA sequences and GenBank database sea sticky false alternating the 16S rDNA sequence of Aeromonas (Pseudoalteromonas mariniglutinosa) 97% - 99% homology. The genetic relationship between the strain CY and 3635T KMM (accession No.: AJ507251) was close, which reached 97.16%. Based on physiological characterization, biochemical test and a partial analysis of the 16S rDNA gene sequence, the bacteria strain CY was identified as Pseudoalteromonas mariniglutinosa which in GenBank sequence number is KT717632. When strain CY are under the conditions of 30°C 250 r/ min shaking culture growth curve, 0 h~ 4 h is the growth lag phase,4 h~60 h is logarithmic phase, 60 h~75 h is stable period and 80 h is the later period of decline. The growth optimum pH and temperature are 7 and 30°C, the optimum NaCl concentration is 20 g / L.
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