摘要生物传感器以其专一性好、易操作、设备简单、测量快速准确的特点，迅速的发展起来，现在已经运用到许多领域当中。但是在如今的研究发展中，生物传感器还是遇到了一些瓶颈，比如信号响应不够强烈，电子传输不够迅速，因此，需要寻找新的材料来克服这些问题。63948

作为近年炙手可热的材料，氧化石墨烯（graphene oxide，GO）被用于本实验，研究它与有机染料邻苯二酚紫（PCV）结合后所发生的结构的变化以及表面特性的变化。本实验中我们将通过紫外-可见分光光度法（UV-vis）和傅里叶变换红外分光光度法（FT-IR）来探究二者复合物的结构变化，通过接触角（contact angle）来探究其亲/疏水的特性。

毕业论文关键词：儿茶酚紫  氧化石墨烯 紫外-可见分光光度法 接触角

Abstract As their specificity is good, easy to operate, simple equipment, fast and accurate measurement of the characteristics, biosensors are now developing rapidly and have been applied to many areas. But in today's research and development, biosensors are still experiencing some bottlenecks, such as signal response was not strong enough and the electron transport was not quick enough. Therefore , we need to find new materials to overcome these problems.

As the very hot material in recent years, graphene oxide(GO) was used in this experiment, we studied the structure changes and changes in surface properties after we combined it with organic dye pyrocatechol violet(PCV). In this study, we will explore the structure changes of the complex by UV - visible spectrophotometry and Fourier transform infrared spectrophotometry, and research the contact angle to explore their hydrophobicity/ hydrophilicity.

Key Words ：PCV  GO  PCV/GO  UV-vis  Contact angle

目录

摘要 2

Abstract 3

1 绪论 4

1.1 儿茶酚紫（邻苯二酚紫）的结构及性质 4

1.2石墨烯的结构及性质 5

1.3 氧化石墨烯 6

1.4 还原态氧化石墨烯 7

1.5 紫外-可见分光光度法 8

1.5.1 方法的特点 8

1.5.2 紫外-可见分光光度计 8

1.5.3 测定法 9

1.6 红外吸收光谱 10

1.7 接触角 10

1.7.1定义 10

1.7.2 接触角的特点 11

1.7.3接触角的测试方法 11

2 实验部分 12

2.1 试剂 12

2.2设备与仪器 12

2.3 还原态氧化石墨烯（RGO）的制备（水合肼法） 12

2.4 儿茶酚紫/还原态氧化石墨烯（PCV/RGO）复合物的制备 13

2.5 紫外-可见吸收光谱的测定 14

2.5.1 PCV溶于不同pH的PBS溶液中的UV-vis吸收光谱 14

2.5.2 PCV溶于不同pH的H2O溶液中的UV-vis吸收光谱 15

2.6  FT-IR红外吸收光谱的测定 15

2.7 接触角 16

3 结果与讨论